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- W2015998286 abstract "3'-O-(5'-phosphoryldeoxycytidyl) phosphorothioate and fluorescent 3'-O-(5'-phosphoryldeoxycytidyl) S-bimane phosphorothioate can be ligated to tRNA by T4 RNA ligase. They are also efficient donors for the enzymatic ligation to oligodeoxynucleotides bearing a 3'-cytidine terminus. Cytidine 3',5'-bisphosphate is also a substrate for the ligation reaction with DNA restriction fragments with a 3'-terminate cytidylic acid residue. Oligo- and polynucleotides with a 3'-phosphorothioate group react readily with electrophiles as exemplified by the reaction with monobromobimane." @default.
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- W2015998286 date "1984-01-01" @default.
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- W2015998286 title "Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase" @default.
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- W2015998286 doi "https://doi.org/10.1093/nar/12.4.1791" @default.
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