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• W2016008957 abstract "Creating different oxygen tensions at a microscale level can be beneficial for studies that investigate oxygen-dependent cellular behavior. Here we present a novel, highly flexible, oxygen tension programmable microfluidic system (oPROMs) capable of generating various uniform oxygen tensions without using complex device structures and multiple gas interconnections. The oPROMs device is coated with a mixture of Glucose oxidase (GOX) and Catalase (CAT) immobilized in a chitosan matrix and then covered by membranes with different thickness. The high glucose medium used in cell culture interacts with the enzyme layer and produces a steady hypoxia inside the wells. Based on different combination of GOX and CAT, oxygen tensions from 0% to 7% were produced." @default.
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• W2016008957 date "2013-06-01" @default.
• W2016008957 modified "2023-09-27" @default.
• W2016008957 title "A novel oxygen tension programmable microfluidic system (oPROMs) for in vitro cell biology studies" @default.
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• W2016008957 doi "https://doi.org/10.1109/transducers.2013.6626790" @default.
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