FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016019757> ?p ?o ?g. }

• W2016019757 endingPage "e0121495" @default.
• W2016019757 startingPage "e0121495" @default.
• W2016019757 abstract "Advances in the “omics” field bring about the need for a high number of good quality samples. Many omics studies take advantage of biobanked samples to meet this need. Most of the laboratory errors occur in the pre-analytical phase. Therefore evidence-based standard operating procedures for the pre-analytical phase as well as markers to distinguish between ‘good’ and ‘bad’ quality samples taking into account the desired downstream analysis are urgently needed. We studied concentration changes of metabolites in serum samples due to pre-storage handling conditions as well as due to repeated freeze-thaw cycles. We collected fasting serum samples and subjected aliquots to up to four freeze-thaw cycles and to pre-storage handling delays of 12, 24 and 36 hours at room temperature (RT) and on wet and dry ice. For each treated aliquot, we quantified 127 metabolites through a targeted metabolomics approach. We found a clear signature of degradation in samples kept at RT. Storage on wet ice led to less pronounced concentration changes. 24 metabolites showed significant concentration changes at RT. In 22 of these, changes were already visible after only 12 hours of storage delay. Especially pronounced were increases in lysophosphatidylcholines and decreases in phosphatidylcholines. We showed that the ratio between the concentrations of these molecule classes could serve as a measure to distinguish between ‘good’ and ‘bad’ quality samples in our study. In contrast, we found quite stable metabolite concentrations during up to four freeze-thaw cycles. We concluded that pre-analytical RT handling of serum samples should be strictly avoided and serum samples should always be handled on wet ice or in cooling devices after centrifugation. Moreover, serum samples should be frozen at or below -80°C as soon as possible after centrifugation." @default.
• W2016019757 created "2016-06-24" @default.
• W2016019757 creator A5001900750 @default.
• W2016019757 creator A5003757583 @default.
• W2016019757 creator A5009921503 @default.
• W2016019757 creator A5013646248 @default.
• W2016019757 creator A5018980466 @default.
• W2016019757 creator A5021048991 @default.
• W2016019757 creator A5029592868 @default.
• W2016019757 creator A5033780024 @default.
• W2016019757 creator A5044923781 @default.
• W2016019757 creator A5061627231 @default.
• W2016019757 creator A5072509338 @default.
• W2016019757 creator A5073377098 @default.
• W2016019757 creator A5074807537 @default.
• W2016019757 creator A5084999936 @default.
• W2016019757 creator A5088727034 @default.
• W2016019757 date "2015-03-30" @default.
• W2016019757 modified "2023-10-18" @default.
• W2016019757 title "Pre-Analytical Sample Quality: Metabolite Ratios as an Intrinsic Marker for Prolonged Room Temperature Exposure of Serum Samples" @default.
• W2016019757 cites W1970045266 @default.
• W2016019757 cites W1997764369 @default.
• W2016019757 cites W2004044531 @default.
• W2016019757 cites W2006141598 @default.
• W2016019757 cites W2017076975 @default.
• W2016019757 cites W2029742084 @default.
• W2016019757 cites W2032058847 @default.
• W2016019757 cites W2035837675 @default.
• W2016019757 cites W2061276809 @default.
• W2016019757 cites W2063733792 @default.
• W2016019757 cites W2066846767 @default.
• W2016019757 cites W2103022797 @default.
• W2016019757 cites W2105537689 @default.
• W2016019757 cites W2106779926 @default.
• W2016019757 cites W2113737470 @default.
• W2016019757 cites W2123454954 @default.
• W2016019757 cites W2124850376 @default.
• W2016019757 cites W2127519720 @default.
• W2016019757 cites W2129246819 @default.
• W2016019757 cites W2133912907 @default.
• W2016019757 cites W2141799067 @default.
• W2016019757 cites W2145665725 @default.
• W2016019757 cites W2150110053 @default.
• W2016019757 cites W2158610710 @default.
• W2016019757 cites W2162521457 @default.
• W2016019757 cites W2325950737 @default.
• W2016019757 cites W2911964244 @default.
• W2016019757 doi "https://doi.org/10.1371/journal.pone.0121495" @default.
• W2016019757 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4379062" @default.
• W2016019757 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25823017" @default.
• W2016019757 hasPublicationYear "2015" @default.
• W2016019757 type Work @default.
• W2016019757 sameAs 2016019757 @default.
• W2016019757 citedByCount "85" @default.
• W2016019757 countsByYear W20160197572015 @default.
• W2016019757 countsByYear W20160197572016 @default.
• W2016019757 countsByYear W20160197572017 @default.
• W2016019757 countsByYear W20160197572018 @default.
• W2016019757 countsByYear W20160197572019 @default.
• W2016019757 countsByYear W20160197572020 @default.
• W2016019757 countsByYear W20160197572021 @default.
• W2016019757 countsByYear W20160197572022 @default.
• W2016019757 countsByYear W20160197572023 @default.
• W2016019757 crossrefType "journal-article" @default.
• W2016019757 hasAuthorship W2016019757A5001900750 @default.
• W2016019757 hasAuthorship W2016019757A5003757583 @default.
• W2016019757 hasAuthorship W2016019757A5009921503 @default.
• W2016019757 hasAuthorship W2016019757A5013646248 @default.
• W2016019757 hasAuthorship W2016019757A5018980466 @default.
• W2016019757 hasAuthorship W2016019757A5021048991 @default.
• W2016019757 hasAuthorship W2016019757A5029592868 @default.
• W2016019757 hasAuthorship W2016019757A5033780024 @default.
• W2016019757 hasAuthorship W2016019757A5044923781 @default.
• W2016019757 hasAuthorship W2016019757A5061627231 @default.
• W2016019757 hasAuthorship W2016019757A5072509338 @default.
• W2016019757 hasAuthorship W2016019757A5073377098 @default.
• W2016019757 hasAuthorship W2016019757A5074807537 @default.
• W2016019757 hasAuthorship W2016019757A5084999936 @default.
• W2016019757 hasAuthorship W2016019757A5088727034 @default.
• W2016019757 hasBestOaLocation W20160197571 @default.
• W2016019757 hasConcept C185592680 @default.
• W2016019757 hasConcept C21565614 @default.
• W2016019757 hasConcept C2777477808 @default.
• W2016019757 hasConcept C43617362 @default.
• W2016019757 hasConcept C55493867 @default.
• W2016019757 hasConceptScore W2016019757C185592680 @default.
• W2016019757 hasConceptScore W2016019757C21565614 @default.
• W2016019757 hasConceptScore W2016019757C2777477808 @default.
• W2016019757 hasConceptScore W2016019757C43617362 @default.
• W2016019757 hasConceptScore W2016019757C55493867 @default.
• W2016019757 hasIssue "3" @default.
• W2016019757 hasLocation W20160197571 @default.
• W2016019757 hasLocation W20160197572 @default.
• W2016019757 hasLocation W20160197573 @default.
• W2016019757 hasLocation W20160197574 @default.
• W2016019757 hasLocation W20160197575 @default.
• W2016019757 hasLocation W20160197576 @default.
• W2016019757 hasLocation W20160197577 @default.

• next