Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016023635> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2016023635 abstract "During mitosis, regulation of protein structures and functions by phosphorylation plays critical roles in orchestrating a series of complex events essential for the cell division process. Tumor-associated microtubule-associated protein (TMAP), also known as cytoskeleton-associated protein 2 (CKAP2), is a novel player in spindle assembly and chromosome segregation. We have previously reported that TMAP is phosphorylated at multiple residues specifically during mitosis. However, the mechanisms and functional importance of phosphorylation at most of the sites identified are currently unknown. Here, we report that TMAP is a novel substrate of the Aurora B kinase. Ser627 of TMAP was specifically phosphorylated by Aurora B both in vitro and in vivo. Ser627 and neighboring conserved residues were strictly required for efficient phosphorylation of TMAP by Aurora B, as even minor amino acid substitutions of the phosphorylation motif significantly diminished the efficiency of the substrate phosphorylation. Nearly all mutations at the phosphorylation motif had dramatic effects on the subcellular localization of TMAP. Instead of being localized to the chromosome region during late mitosis, the mutants remained associated with microtubules and centrosomes throughout mitosis. However, the changes in the subcellular localization of these mutants could not be completely explained by the phosphorylation status on Ser627. Our findings suggest that the motif surrounding Ser627 ((625) RRSRRL (630)) is a critical part of a functionally important sequence motif which not only governs the kinase-substrate recognition, but also regulates the subcellular localization of TMAP during mitosis." @default.
- W2016023635 created "2016-06-24" @default.
- W2016023635 creator A5018940400 @default.
- W2016023635 creator A5049569893 @default.
- W2016023635 creator A5056462380 @default.
- W2016023635 creator A5069465297 @default.
- W2016023635 creator A5077941411 @default.
- W2016023635 date "2010-05-15" @default.
- W2016023635 modified "2023-09-27" @default.
- W2016023635 title "Specific primary sequence requirements for Aurora B kinase-mediated phosphorylation and subcellular localization of TMAP during mitosis" @default.
- W2016023635 doi "https://doi.org/10.4161/cc.9.10.11753" @default.
- W2016023635 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20458174" @default.
- W2016023635 hasPublicationYear "2010" @default.
- W2016023635 type Work @default.
- W2016023635 sameAs 2016023635 @default.
- W2016023635 citedByCount "7" @default.
- W2016023635 countsByYear W20160236352012 @default.
- W2016023635 countsByYear W20160236352013 @default.
- W2016023635 countsByYear W20160236352014 @default.
- W2016023635 countsByYear W20160236352017 @default.
- W2016023635 crossrefType "journal-article" @default.
- W2016023635 hasAuthorship W2016023635A5018940400 @default.
- W2016023635 hasAuthorship W2016023635A5049569893 @default.
- W2016023635 hasAuthorship W2016023635A5056462380 @default.
- W2016023635 hasAuthorship W2016023635A5069465297 @default.
- W2016023635 hasAuthorship W2016023635A5077941411 @default.
- W2016023635 hasBestOaLocation W20160236351 @default.
- W2016023635 hasConcept C104317684 @default.
- W2016023635 hasConcept C104551158 @default.
- W2016023635 hasConcept C11960822 @default.
- W2016023635 hasConcept C1491633281 @default.
- W2016023635 hasConcept C184235292 @default.
- W2016023635 hasConcept C2778354886 @default.
- W2016023635 hasConcept C30481170 @default.
- W2016023635 hasConcept C47432576 @default.
- W2016023635 hasConcept C55493867 @default.
- W2016023635 hasConcept C85813293 @default.
- W2016023635 hasConcept C86803240 @default.
- W2016023635 hasConcept C87325107 @default.
- W2016023635 hasConcept C93304396 @default.
- W2016023635 hasConcept C95444343 @default.
- W2016023635 hasConcept C97029542 @default.
- W2016023635 hasConceptScore W2016023635C104317684 @default.
- W2016023635 hasConceptScore W2016023635C104551158 @default.
- W2016023635 hasConceptScore W2016023635C11960822 @default.
- W2016023635 hasConceptScore W2016023635C1491633281 @default.
- W2016023635 hasConceptScore W2016023635C184235292 @default.
- W2016023635 hasConceptScore W2016023635C2778354886 @default.
- W2016023635 hasConceptScore W2016023635C30481170 @default.
- W2016023635 hasConceptScore W2016023635C47432576 @default.
- W2016023635 hasConceptScore W2016023635C55493867 @default.
- W2016023635 hasConceptScore W2016023635C85813293 @default.
- W2016023635 hasConceptScore W2016023635C86803240 @default.
- W2016023635 hasConceptScore W2016023635C87325107 @default.
- W2016023635 hasConceptScore W2016023635C93304396 @default.
- W2016023635 hasConceptScore W2016023635C95444343 @default.
- W2016023635 hasConceptScore W2016023635C97029542 @default.
- W2016023635 hasLocation W20160236351 @default.
- W2016023635 hasLocation W20160236352 @default.
- W2016023635 hasOpenAccess W2016023635 @default.
- W2016023635 hasPrimaryLocation W20160236351 @default.
- W2016023635 hasRelatedWork W177734819 @default.
- W2016023635 hasRelatedWork W1838948892 @default.
- W2016023635 hasRelatedWork W1966951204 @default.
- W2016023635 hasRelatedWork W2002648866 @default.
- W2016023635 hasRelatedWork W2040633485 @default.
- W2016023635 hasRelatedWork W2043051103 @default.
- W2016023635 hasRelatedWork W2047316016 @default.
- W2016023635 hasRelatedWork W2048131815 @default.
- W2016023635 hasRelatedWork W2051717153 @default.
- W2016023635 hasRelatedWork W2055814913 @default.
- W2016023635 hasRelatedWork W2056132322 @default.
- W2016023635 hasRelatedWork W2064795388 @default.
- W2016023635 hasRelatedWork W2065937550 @default.
- W2016023635 hasRelatedWork W2098962955 @default.
- W2016023635 hasRelatedWork W2104063044 @default.
- W2016023635 hasRelatedWork W2112064734 @default.
- W2016023635 hasRelatedWork W2125754609 @default.
- W2016023635 hasRelatedWork W2130364109 @default.
- W2016023635 hasRelatedWork W2138215378 @default.
- W2016023635 hasRelatedWork W2566653121 @default.
- W2016023635 isParatext "false" @default.
- W2016023635 isRetracted "false" @default.
- W2016023635 magId "2016023635" @default.
- W2016023635 workType "article" @default.