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• W2016024916 abstract "The mechanism of caspase-3-dependent apoptosis induced by photodynamic therapy (PDT) of cultured Chinese hamster V79 cells with pheophorbide a (PPa) was investigated. The PPa-PDT induced rapid apoptosis within 30 min after irradiation of cells. This apoptosis was inhibited by the 1O2 quencher N3-and caspase-3 inhibitor acetyl-Asp-Glu-Val-Asp-aldehyde, suggesting that 1O2 activated caspase-3 and then caused apoptosis. The intracellular calcium [Ca2+]1 chelator (acetoxymethyl)-l,2-bis(o-aminophenoxy)ethane N,N,N',N,'-tetraacetic acid (BAPTA-AM) and the cyclic adenosine monophosphate (cAMP)-increasing agent forskolin also inhibited not only the PPa-PDT-induced activation of caspase-3 but also apoptosis in V79 cells. Furthermore, PPa-PDT-induced cytochrome c release from mitochondria was found to be inhibited by the treatment with BAPTA-AM but not forskolin. These results indicated that [Ca2+]1 and cAMP independently serve as regulators for PPa-PDT-induced apoptosis in the upstream of caspase-3." @default.
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• W2016024916 date "1999-10-01" @default.
• W2016024916 modified "2023-10-07" @default.
• W2016024916 title "Effects of BAPTA-AM and Forskolin on Apoptosis and Cytochrome c Release in Photosensitized Chinese Hamster V79 Cells" @default.
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• W2016024916 doi "https://doi.org/10.1111/j.1751-1097.1999.tb08265.x" @default.
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