Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016035325> ?p ?o ?g. }
- W2016035325 abstract "Abstract We have previously reported the presence of an apyrase in Mimosa pudica. However, only limited information is available for this enzyme. Thus, in this study, the apyrase was purified to homogeneity. The purified enzyme had a molecular mass of around 67 kD and was able to hydrolyze both nucleotide triphosphate and nucleotide diphosphate as substrates. The ratio of ATP to ADP hydrolysis velocity of the purified protein was 0.01 in the presence of calcium ion, showing extremely high substrate specificity toward ADP. Thus, we designated this novel apyrase as MP67. A cDNA clone of MP67 was obtained using primers designed from the amino acid sequence of trypsin-digested fragments of the protein. In addition, rapid amplification of cDNA ends-polymerase chain reaction was performed to clone a conventional apyrase (MpAPY2). Comparison of the deduced amino acid sequences showed that MP67 is similar to ecto-apyrases; however, it was distinct from conventional apyrase based on phylogenetic classification. MP67 and MpAPY2 were expressed in Escherichia coli, and the recombinant proteins were purified. The recombinant MP67 showed high substrate specificity toward ADP rather than ATP. A polyclonal antibody raised against the recombinant MP67 was used to examine the tissue distribution and localization of native MP67 in the plant. The results showed that MP67 was ubiquitously distributed in various tissues, most abundantly in leaves, and was localized to plasma membranes. Thus, MP67 is a novel ecto-apyrase with extremely high substrate specificity for ADP." @default.
- W2016035325 created "2016-06-24" @default.
- W2016035325 creator A5015955730 @default.
- W2016035325 creator A5022379278 @default.
- W2016035325 creator A5031980607 @default.
- W2016035325 creator A5052036696 @default.
- W2016035325 creator A5060936886 @default.
- W2016035325 creator A5072730306 @default.
- W2016035325 date "2011-07-25" @default.
- W2016035325 modified "2023-09-22" @default.
- W2016035325 title "Purification and Biochemical Characterization of a Novel Ecto-Apyrase, MP67, from Mimosa pudica " @default.
- W2016035325 cites W1519164650 @default.
- W2016035325 cites W1570916029 @default.
- W2016035325 cites W1591309477 @default.
- W2016035325 cites W1964267582 @default.
- W2016035325 cites W1965322483 @default.
- W2016035325 cites W1968031524 @default.
- W2016035325 cites W1971851348 @default.
- W2016035325 cites W1990173893 @default.
- W2016035325 cites W1991477667 @default.
- W2016035325 cites W2002451448 @default.
- W2016035325 cites W2003255160 @default.
- W2016035325 cites W2004225521 @default.
- W2016035325 cites W2006177579 @default.
- W2016035325 cites W2008259032 @default.
- W2016035325 cites W2008772223 @default.
- W2016035325 cites W2009657322 @default.
- W2016035325 cites W2014959551 @default.
- W2016035325 cites W2016797897 @default.
- W2016035325 cites W2023811960 @default.
- W2016035325 cites W2029018989 @default.
- W2016035325 cites W2030044405 @default.
- W2016035325 cites W2037102204 @default.
- W2016035325 cites W2052913130 @default.
- W2016035325 cites W2054974598 @default.
- W2016035325 cites W2057703795 @default.
- W2016035325 cites W2069524025 @default.
- W2016035325 cites W2076174511 @default.
- W2016035325 cites W2080884402 @default.
- W2016035325 cites W2082539152 @default.
- W2016035325 cites W2096238127 @default.
- W2016035325 cites W2096363719 @default.
- W2016035325 cites W2097382368 @default.
- W2016035325 cites W2099317945 @default.
- W2016035325 cites W2100430794 @default.
- W2016035325 cites W2100837269 @default.
- W2016035325 cites W2101755974 @default.
- W2016035325 cites W2104244040 @default.
- W2016035325 cites W2105898536 @default.
- W2016035325 cites W2106702564 @default.
- W2016035325 cites W2110813692 @default.
- W2016035325 cites W2111301002 @default.
- W2016035325 cites W2117919289 @default.
- W2016035325 cites W2140069334 @default.
- W2016035325 cites W2155025856 @default.
- W2016035325 cites W2155827187 @default.
- W2016035325 cites W2168092917 @default.
- W2016035325 cites W4293247451 @default.
- W2016035325 cites W79102471 @default.
- W2016035325 doi "https://doi.org/10.1104/pp.111.180414" @default.
- W2016035325 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3165892" @default.
- W2016035325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21788364" @default.
- W2016035325 hasPublicationYear "2011" @default.
- W2016035325 type Work @default.
- W2016035325 sameAs 2016035325 @default.
- W2016035325 citedByCount "8" @default.
- W2016035325 countsByYear W20160353252012 @default.
- W2016035325 countsByYear W20160353252013 @default.
- W2016035325 countsByYear W20160353252014 @default.
- W2016035325 countsByYear W20160353252015 @default.
- W2016035325 countsByYear W20160353252019 @default.
- W2016035325 countsByYear W20160353252020 @default.
- W2016035325 crossrefType "journal-article" @default.
- W2016035325 hasAuthorship W2016035325A5015955730 @default.
- W2016035325 hasAuthorship W2016035325A5022379278 @default.
- W2016035325 hasAuthorship W2016035325A5031980607 @default.
- W2016035325 hasAuthorship W2016035325A5052036696 @default.
- W2016035325 hasAuthorship W2016035325A5060936886 @default.
- W2016035325 hasAuthorship W2016035325A5072730306 @default.
- W2016035325 hasBestOaLocation W20160353251 @default.
- W2016035325 hasConcept C104317684 @default.
- W2016035325 hasConcept C153911025 @default.
- W2016035325 hasConcept C167625842 @default.
- W2016035325 hasConcept C181199279 @default.
- W2016035325 hasConcept C187882448 @default.
- W2016035325 hasConcept C2779508331 @default.
- W2016035325 hasConcept C40767141 @default.
- W2016035325 hasConcept C512185932 @default.
- W2016035325 hasConcept C515207424 @default.
- W2016035325 hasConcept C55493867 @default.
- W2016035325 hasConcept C58121356 @default.
- W2016035325 hasConcept C86803240 @default.
- W2016035325 hasConceptScore W2016035325C104317684 @default.
- W2016035325 hasConceptScore W2016035325C153911025 @default.
- W2016035325 hasConceptScore W2016035325C167625842 @default.
- W2016035325 hasConceptScore W2016035325C181199279 @default.
- W2016035325 hasConceptScore W2016035325C187882448 @default.
- W2016035325 hasConceptScore W2016035325C2779508331 @default.
- W2016035325 hasConceptScore W2016035325C40767141 @default.
- W2016035325 hasConceptScore W2016035325C512185932 @default.