Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016039733> ?p ?o ?g. }
- W2016039733 endingPage "736" @default.
- W2016039733 startingPage "728" @default.
- W2016039733 abstract "Saponin detoxification enzymes from pathogenic fungi are involved in the infection process of their host plants. Fusarium oxysporum f. sp lycopersici, a tomato pathogen, produces the tomatinase enzyme Tom1, which degrades alpha-tomatine to less toxic derivates. To study the role of the tom1 gene in the virulence of F. oxysporum, we performed targeted disruption and overexpression of the gene. The infection process of tomato plants inoculated with transformants constitutively producing Tom1 resulted in an increase of symptom development. By contrast, tomato plants infected with the knockout mutants showed a delay in the disease process, indicating that Tom1, although not essential for pathogenicity, is required for the full virulence of F. oxysporum. Total tomatinase activity in the disrupted strains was reduced only 25%, leading to beta(2)-tomatine as the main hydrolysis product of the saponin in vitro. In silico analysis of the F. oxysporum genome revealed the existence of four additional putative tomatinase genes with identities to tomatinases from family 3 of glycosyl hydrolases. These might be responsible for the remaining tomatinase activity in the Deltatom1 mutants. Our results indicate that detoxification of alpha-tomatine in F. oxysporum is carried out by several tomatinase activities, suggesting the importance of these enzymes during the infection process." @default.
- W2016039733 created "2016-06-24" @default.
- W2016039733 creator A5002513468 @default.
- W2016039733 creator A5049720279 @default.
- W2016039733 creator A5082308268 @default.
- W2016039733 date "2008-06-01" @default.
- W2016039733 modified "2023-10-17" @default.
- W2016039733 title "Tomatinase from <i>Fusarium oxysporum</i> f. sp. <i>lycopersici</i> Is Required for Full Virulence on Tomato Plants" @default.
- W2016039733 cites W1502826000 @default.
- W2016039733 cites W1509611593 @default.
- W2016039733 cites W1511463189 @default.
- W2016039733 cites W1521577395 @default.
- W2016039733 cites W1543968842 @default.
- W2016039733 cites W1563693268 @default.
- W2016039733 cites W1590172349 @default.
- W2016039733 cites W1772341111 @default.
- W2016039733 cites W1964158438 @default.
- W2016039733 cites W1971003087 @default.
- W2016039733 cites W1976408627 @default.
- W2016039733 cites W1987574543 @default.
- W2016039733 cites W1988168397 @default.
- W2016039733 cites W1994731031 @default.
- W2016039733 cites W1995309636 @default.
- W2016039733 cites W1995653318 @default.
- W2016039733 cites W1996535761 @default.
- W2016039733 cites W199814218 @default.
- W2016039733 cites W1998964253 @default.
- W2016039733 cites W2006697820 @default.
- W2016039733 cites W2009674410 @default.
- W2016039733 cites W2012648892 @default.
- W2016039733 cites W2027312376 @default.
- W2016039733 cites W2035747469 @default.
- W2016039733 cites W2037183324 @default.
- W2016039733 cites W2041105433 @default.
- W2016039733 cites W2045951268 @default.
- W2016039733 cites W2049872306 @default.
- W2016039733 cites W2050687766 @default.
- W2016039733 cites W2055043387 @default.
- W2016039733 cites W2063033678 @default.
- W2016039733 cites W2072836964 @default.
- W2016039733 cites W2075120508 @default.
- W2016039733 cites W2087928696 @default.
- W2016039733 cites W2091302656 @default.
- W2016039733 cites W2103231201 @default.
- W2016039733 cites W2103546861 @default.
- W2016039733 cites W2106882534 @default.
- W2016039733 cites W2108313755 @default.
- W2016039733 cites W2110462211 @default.
- W2016039733 cites W2111830072 @default.
- W2016039733 cites W2117059491 @default.
- W2016039733 cites W2117512575 @default.
- W2016039733 cites W2122524572 @default.
- W2016039733 cites W2126059415 @default.
- W2016039733 cites W2126371728 @default.
- W2016039733 cites W2144073967 @default.
- W2016039733 cites W2166089275 @default.
- W2016039733 cites W221917755 @default.
- W2016039733 cites W2335694731 @default.
- W2016039733 cites W251520332 @default.
- W2016039733 cites W4229493649 @default.
- W2016039733 doi "https://doi.org/10.1094/mpmi-21-6-0728" @default.
- W2016039733 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18624637" @default.
- W2016039733 hasPublicationYear "2008" @default.
- W2016039733 type Work @default.
- W2016039733 sameAs 2016039733 @default.
- W2016039733 citedByCount "67" @default.
- W2016039733 countsByYear W20160397332012 @default.
- W2016039733 countsByYear W20160397332013 @default.
- W2016039733 countsByYear W20160397332014 @default.
- W2016039733 countsByYear W20160397332015 @default.
- W2016039733 countsByYear W20160397332016 @default.
- W2016039733 countsByYear W20160397332017 @default.
- W2016039733 countsByYear W20160397332018 @default.
- W2016039733 countsByYear W20160397332019 @default.
- W2016039733 countsByYear W20160397332020 @default.
- W2016039733 countsByYear W20160397332021 @default.
- W2016039733 countsByYear W20160397332022 @default.
- W2016039733 countsByYear W20160397332023 @default.
- W2016039733 crossrefType "journal-article" @default.
- W2016039733 hasAuthorship W2016039733A5002513468 @default.
- W2016039733 hasAuthorship W2016039733A5049720279 @default.
- W2016039733 hasAuthorship W2016039733A5082308268 @default.
- W2016039733 hasBestOaLocation W20160397331 @default.
- W2016039733 hasConcept C104317684 @default.
- W2016039733 hasConcept C142724271 @default.
- W2016039733 hasConcept C143065580 @default.
- W2016039733 hasConcept C204787440 @default.
- W2016039733 hasConcept C2776460866 @default.
- W2016039733 hasConcept C2778564307 @default.
- W2016039733 hasConcept C2779430813 @default.
- W2016039733 hasConcept C54355233 @default.
- W2016039733 hasConcept C59822182 @default.
- W2016039733 hasConcept C60987743 @default.
- W2016039733 hasConcept C71924100 @default.
- W2016039733 hasConcept C86803240 @default.
- W2016039733 hasConcept C89423630 @default.
- W2016039733 hasConceptScore W2016039733C104317684 @default.
- W2016039733 hasConceptScore W2016039733C142724271 @default.