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- W2016040218 abstract "The potential of immobilized pH gradients has been explored as a means of classifying the common phenotypes of Gc in human plasma and bloodstains of varying ages. A comparison was made between the degree of separation of the common Gc phenotypes in plasma using three isoelectric focusing methods: two ultra-thin (0·15 mm) techniques using pH 4–6 Ampholines, one with the addition of a separator to narrow the pH gradient, and a third using an immobilized pH gradient containing Immobilines in a 0·5 mm gel. The greatest degree of separation of the common Gc phenotypes was achieved with the immobilized pH gradients (Immobiline gels). Using these gels, Gc phenotypes were detected in 7-month-old plasma samples by direct protein staining and in bloodstains of the same age by immunofixation with an antibody specific for human Gc. The duration of immunofixation was found to be important for the detection of Gc phenotypes in bloodstains when separated on Immobiline gels. As a final assessment, the results of a blind trial organised by the Home Office Forensic Science Laboratory, Birmingham, indicate that the technique is generally reliable and allows typing in over 80% of bloodstains less than 7 months old, although some fresh stains could not be typed." @default.
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- W2016040218 date "1984-09-01" @default.
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- W2016040218 title "Analysis of the Common Genetic Variants of the Human Gc System in Plasma and Bloodstains by Isoelectric Focusing in Immobilized pH Gradients" @default.
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- W2016040218 doi "https://doi.org/10.1016/s0015-7368(84)72331-0" @default.
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