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• W2016043735 abstract "We investigated the interaction of HIV immune complexes (HIV IC) with mononuclear cells from lymph nodes and blood. While antibody alone did not affect binding of HIV IC to mononuclear cells, antibody plus complement increased binding by as much as 10-fold and complement alone also increased binding slightly. Most of the increased binding of HIV IC to mononuclear cells was blocked by heat-inactivation of complement and by OKB7 monoclonal antibody, indicating that virus binding was to CR2 on B cells. A similar pattern of antibody and complement dependence for binding of HIV IC was observed with two model systems; Raji and Arent B-cell lines. Most of the HIV IC that bound to lymph node cells were not internalized, but remained on the cell surface and were gradually released. However, even after 48 hr some HIV IC could be detected bound to cells. Under certain conditions, HIV IC were infectious for T cells if bound to B cells but not infectious if added directly to T cells. Additionally, HIV IC bound to B cells led to higher virus replication. These studies show that B lymphocytes from blood and lymph nodes can transfer infectious HIV IC to T cells." @default.
• W2016043735 created "2016-06-24" @default.
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• W2016043735 date "1999-04-01" @default.
• W2016043735 modified "2023-10-16" @default.
• W2016043735 title "B lymphocytes in lymph nodes and peripheral blood are important for binding immune complexes containing HIV‐1" @default.
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• W2016043735 doi "https://doi.org/10.1046/j.1365-2567.1999.00304.x" @default.
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