FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016048823> ?p ?o ?g. }

• W2016048823 endingPage "1233" @default.
• W2016048823 startingPage "1223" @default.
• W2016048823 abstract "A human caput epididymidal cDNA, HE2C, was cloned based on its homology to the known chimpanzee counterpart, suggesting that the encoded β-defensin-like peptide represented a conserved component of the innate epididymidal epithelial defense system in primates. An approximately 6 kDa HE2- related peptide was co-purified together with other HE2 isoforms from human seminal plasma by affinity chromatography. By its antibody reactivity as shown by Western blot analysis, this peptide was distinct from the more abundant HE2 isoforms and was concluded to correspond to HE2C. Similar to other HE2-encoded isoforms, the endogenous HE2C was proteolytically processed from a larger precursor by a furin-like prohormone convertase. This was confirmed by N-terminal sequencing. In order to study the structural and functional properties of HE2C it was recombinantly expressed in insect cells. Post-translational processing also occurred within these cells, yielding the mature processed HE2C peptide. Correct disulfide bonding of the recHE2C peptide was shown by p-aminophenylarsineoxide(PAPAO)-agarose binding assay. Purified recHE2C strongly bound to Escherichia coli DH5α and Bacillus subtilis; however, it did not exhibit microbicidal activity when tested in a radial diffusion assay against these bacteria. Different from the previously described β-defensins, the mature HE2C peptide has an anionic pI and an algebraic net charge of −1. Also, it lacks the amphipathic transitions, which, according to the Shai-Matzusaki-Huang model, are prerequisite for the membranolytic activity of antimicrobial peptides." @default.
• W2016048823 created "2016-06-24" @default.
• W2016048823 creator A5002101731 @default.
• W2016048823 creator A5054826755 @default.
• W2016048823 creator A5090719337 @default.
• W2016048823 date "2004-08-01" @default.
• W2016048823 modified "2023-10-01" @default.
• W2016048823 title "SPAG11/isoform HE2C, an atypical anionic β-defensin-like peptide" @default.
• W2016048823 cites W1662185404 @default.
• W2016048823 cites W1664985344 @default.
• W2016048823 cites W1968233064 @default.
• W2016048823 cites W1974056949 @default.
• W2016048823 cites W1975085160 @default.
• W2016048823 cites W1975304761 @default.
• W2016048823 cites W1979891359 @default.
• W2016048823 cites W1991845143 @default.
• W2016048823 cites W1997656734 @default.
• W2016048823 cites W2004372657 @default.
• W2016048823 cites W2005637156 @default.
• W2016048823 cites W2007807255 @default.
• W2016048823 cites W2011612201 @default.
• W2016048823 cites W2011654657 @default.
• W2016048823 cites W2023604719 @default.
• W2016048823 cites W2045777881 @default.
• W2016048823 cites W2054602599 @default.
• W2016048823 cites W2063951978 @default.
• W2016048823 cites W2067799321 @default.
• W2016048823 cites W2080069132 @default.
• W2016048823 cites W2081219821 @default.
• W2016048823 cites W2090042814 @default.
• W2016048823 cites W2098306468 @default.
• W2016048823 cites W2105437547 @default.
• W2016048823 cites W2107803079 @default.
• W2016048823 cites W2112142691 @default.
• W2016048823 cites W2121501557 @default.
• W2016048823 cites W2122742871 @default.
• W2016048823 cites W2124508823 @default.
• W2016048823 cites W2131036658 @default.
• W2016048823 cites W2139412501 @default.
• W2016048823 cites W2158789628 @default.
• W2016048823 cites W2162558901 @default.
• W2016048823 cites W2162819583 @default.
• W2016048823 cites W4211081371 @default.
• W2016048823 cites W4240946843 @default.
• W2016048823 cites W4251766200 @default.
• W2016048823 doi "https://doi.org/10.1016/j.peptides.2004.05.016" @default.
• W2016048823 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15350689" @default.
• W2016048823 hasPublicationYear "2004" @default.
• W2016048823 type Work @default.
• W2016048823 sameAs 2016048823 @default.
• W2016048823 citedByCount "22" @default.
• W2016048823 countsByYear W20160488232013 @default.
• W2016048823 countsByYear W20160488232014 @default.
• W2016048823 countsByYear W20160488232015 @default.
• W2016048823 countsByYear W20160488232016 @default.
• W2016048823 countsByYear W20160488232023 @default.
• W2016048823 crossrefType "journal-article" @default.
• W2016048823 hasAuthorship W2016048823A5002101731 @default.
• W2016048823 hasAuthorship W2016048823A5054826755 @default.
• W2016048823 hasAuthorship W2016048823A5090719337 @default.
• W2016048823 hasConcept C104317684 @default.
• W2016048823 hasConcept C10858879 @default.
• W2016048823 hasConcept C153911025 @default.
• W2016048823 hasConcept C167625842 @default.
• W2016048823 hasConcept C187882448 @default.
• W2016048823 hasConcept C2776415932 @default.
• W2016048823 hasConcept C2777272437 @default.
• W2016048823 hasConcept C2779281246 @default.
• W2016048823 hasConcept C523546767 @default.
• W2016048823 hasConcept C53345823 @default.
• W2016048823 hasConcept C540938839 @default.
• W2016048823 hasConcept C54355233 @default.
• W2016048823 hasConcept C547475151 @default.
• W2016048823 hasConcept C55493867 @default.
• W2016048823 hasConcept C86803240 @default.
• W2016048823 hasConceptScore W2016048823C104317684 @default.
• W2016048823 hasConceptScore W2016048823C10858879 @default.
• W2016048823 hasConceptScore W2016048823C153911025 @default.
• W2016048823 hasConceptScore W2016048823C167625842 @default.
• W2016048823 hasConceptScore W2016048823C187882448 @default.
• W2016048823 hasConceptScore W2016048823C2776415932 @default.
• W2016048823 hasConceptScore W2016048823C2777272437 @default.
• W2016048823 hasConceptScore W2016048823C2779281246 @default.
• W2016048823 hasConceptScore W2016048823C523546767 @default.
• W2016048823 hasConceptScore W2016048823C53345823 @default.
• W2016048823 hasConceptScore W2016048823C540938839 @default.
• W2016048823 hasConceptScore W2016048823C54355233 @default.
• W2016048823 hasConceptScore W2016048823C547475151 @default.
• W2016048823 hasConceptScore W2016048823C55493867 @default.
• W2016048823 hasConceptScore W2016048823C86803240 @default.
• W2016048823 hasIssue "8" @default.
• W2016048823 hasLocation W20160488231 @default.
• W2016048823 hasLocation W20160488232 @default.
• W2016048823 hasOpenAccess W2016048823 @default.
• W2016048823 hasPrimaryLocation W20160488231 @default.
• W2016048823 hasRelatedWork W1786536704 @default.
• W2016048823 hasRelatedWork W1984646339 @default.
• W2016048823 hasRelatedWork W2002193923 @default.

• next