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- W2016068651 abstract "To evaluate a slow freezing method for whole ovary cryopreservation by evaluating effects of added cryoprotectant.Sheep ovaries were isolated during surgery, flushed with either Ringer-Acetate or dimethylsulphoxide and cryopreserved by slow freezing. After rapid thawing, viability was assessed by ovarian in vitro perfusion, cell culture, histology and fluorescent live-dead assay.Production of cyclic AMP and progesterone was slightly higher in the dimethylsulphoxide group. Cultured ovarian cells from dimethylsulphoxide-preserved ovaries secreted larger amounts of progesterone than cells from Ringer-Acetate preserved. Light microscopy of ovarian biopsies obtained after perfusion, revealed well-preserved tissue in the dimethysulphoxide group but not in the Ringer-Acetate group. The density of small follicles and ovarian cell viability were higher in dimethysulphoxide ovaries compared to Ringer-Acetate ovaries.Equilibrium with its protective effect can be achieved by slow freezing protocol, with an additional protective effect by the presence of dimethylsulphoxide." @default.
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- W2016068651 date "2010-09-15" @default.
- W2016068651 modified "2023-10-14" @default.
- W2016068651 title "Whole sheep ovary cryopreservation: evaluation of a slow freezing protocol with dimethylsulphoxide" @default.
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- W2016068651 doi "https://doi.org/10.1007/s10815-010-9477-5" @default.
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