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- W2016073283 abstract "Maturation has been induced in full grown oocytes of Xenopus laevis by incubation in progesterone, and the ultrastructure of their nucleoli has been examined by electron microscopy using the chromatin spreading technique. We show that in this species numerous extrachromosomal nucleoli maintain high levels of transcription of rDNA for up to 200 min after the application of the hormone, i.e., shortly before germinal vesicle breakdown. Transcription has been identified as normal arrays of matrix units containing densely packed transcriptional complexes. In addition to normal-sized arrays of gradients of nascent RNP fibrils, as typical of active pre-rRNA genes, a number of unusual structures are described which include situations of sparse coverage of lateral fibrils in some matrix units, indicative of reduced frequencies of initiation events. The observations are discussed in relation to the time course of nucleolar gene inactivation described in oocytes of other amphibian species, as well as in relation to inactivation of chromosomal genes characteristic of this step of meiotic prophase I." @default.
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- W2016073283 date "1981-12-01" @default.
- W2016073283 modified "2023-10-16" @default.
- W2016073283 title "Patterns of Transcriptional Activity of Nucleolar Genes During Progesterone-Induced Maturation of Oocytes of Xenopus laevis" @default.
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- W2016073283 doi "https://doi.org/10.1111/j.1432-0436.1981.tb01153.x" @default.
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