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• W2016076338 abstract "DNA polymerases have been isolated from muscle and melanoma tissues of Xiphophorus, which are similar to retroviral RNA-dependent DNA polymerases as they prefer RNA to DNA templates. They appear to associate with submicroscopic structures which exhibit a density of about 1.13 g/ml after sucrose-density-gradient centrifugation. The RNA-dependent-DNA-polymerase-like enzymes could be separated from the DNA-dependent DNA polymerases by DEAE-cellulose chromatography. Further purification on phosphocellulose revealed that the muscle enzyme eluted at the void volume and at about 0.6 M KCl, whereas most of the melanoma enzyme eluted at 0.1 M KCl. Comparison of the template primer specificities of the muscle and melanoma enzymes with those of known DNA polymerases showed obvious similarities to the RNA-dependent DNA polymerase isolated from Rous sarcoma virus." @default.
• W2016076338 created "2016-06-24" @default.
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• W2016076338 date "1990-03-01" @default.
• W2016076338 modified "2023-09-27" @default.
• W2016076338 title "Detection of RNA-dependent DNA polymerase activity in the Xiphophorus melanoma system" @default.
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• W2016076338 doi "https://doi.org/10.1111/j.1432-1033.1990.tb15403.x" @default.
• W2016076338 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1690649" @default.
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