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- W2016081374 abstract "Hybridoma antibodies to Drosophila melanogaster soluble yolk proteins (YPs) were developed by both in vivo and in vitro immunizations followed by the fusion of SP2/0-Ag14 cells and splenocytes of BALB/c mice. Rabbit antiserum was made female specific by affinity column with male proteins as ligand. The binding sites of these hybridoma antibodies and rabbit antibodies towards different YP components were identified with a combination of gel electrophoresis, Western blotting and immunohistochemical staining. A double antibody sandwich enzyme-linked immunosorbent assay was developed with monoclonal antibodies from 2 cell lines and alkaline phosphatase labelled rabbit polyclonal antibodies as primary and secondary antibodies respectively. Yolk polypeptide levels in the haemolymph can be monitored in individual insect samples." @default.
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- W2016081374 date "1986-01-01" @default.
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- W2016081374 title "Hybridoma antibodies as specific probes to Drosophila melanogaster yolk polypeptides" @default.
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- W2016081374 doi "https://doi.org/10.1016/0020-1790(86)90115-0" @default.
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