FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016084390> ?p ?o ?g. }

• W2016084390 endingPage "42" @default.
• W2016084390 startingPage "33" @default.
• W2016084390 abstract "Quantitation of HIV-1 specific RNA and DNA is pivotal to understanding the pathophysiology of HIV-1 diseases. A method has been developed for quantitation of HIV-1 DNA/RNA by real-time PCR using a unique fluorogenic primer-probe adduct known as scorpion. The probe hybridises to the extension of the adjoining primer intramolecularly, a process kinetically and thermodynamically more favourable than the conventional bimolecular probe-target hybridisation. Data presented in this paper indicate that the scorpion assay is extremely robust and is quite comparable to beacon-based assays. The scorpion assay is also comparable to quantitative competitive PCR (QC--PCR) assays but requires only a fraction of time and effort. Additionally, the dynamic range of the scorpion assay is several log-fold higher than the conventional end point PCR assays. As few as ten copies of vDNA can be detected in the presence of a large excess of exogenously added genomic DNA. Limiting dilution analysis indicates that the assay is capable of detecting a single copy of the viral template. Thus, the scorpion assay presents a specific and sensitive approach for quantitation of DNA/RNA templates by real-time PCR." @default.
• W2016084390 created "2016-06-24" @default.
• W2016084390 creator A5001211705 @default.
• W2016084390 creator A5065542731 @default.
• W2016084390 creator A5077050364 @default.
• W2016084390 date "2001-04-01" @default.
• W2016084390 modified "2023-09-24" @default.
• W2016084390 title "Quantitation of HIV-1 by real-time PCR with a unique fluorogenic probe" @default.
• W2016084390 cites W1559760142 @default.
• W2016084390 cites W1569947224 @default.
• W2016084390 cites W1569955072 @default.
• W2016084390 cites W1824323511 @default.
• W2016084390 cites W1962521045 @default.
• W2016084390 cites W1988835951 @default.
• W2016084390 cites W1991338345 @default.
• W2016084390 cites W1998582864 @default.
• W2016084390 cites W2002083514 @default.
• W2016084390 cites W2002904896 @default.
• W2016084390 cites W2018466980 @default.
• W2016084390 cites W2026710381 @default.
• W2016084390 cites W2029866053 @default.
• W2016084390 cites W2036890038 @default.
• W2016084390 cites W2066409312 @default.
• W2016084390 cites W2082588492 @default.
• W2016084390 cites W2084393734 @default.
• W2016084390 cites W2096479809 @default.
• W2016084390 cites W2107747866 @default.
• W2016084390 cites W2126838772 @default.
• W2016084390 cites W2137671068 @default.
• W2016084390 cites W2153977602 @default.
• W2016084390 cites W2164578725 @default.
• W2016084390 doi "https://doi.org/10.1016/s0166-0934(00)00288-3" @default.
• W2016084390 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11311341" @default.
• W2016084390 hasPublicationYear "2001" @default.
• W2016084390 type Work @default.
• W2016084390 sameAs 2016084390 @default.
• W2016084390 citedByCount "53" @default.
• W2016084390 countsByYear W20160843902012 @default.
• W2016084390 countsByYear W20160843902013 @default.
• W2016084390 countsByYear W20160843902014 @default.
• W2016084390 countsByYear W20160843902015 @default.
• W2016084390 countsByYear W20160843902016 @default.
• W2016084390 countsByYear W20160843902017 @default.
• W2016084390 countsByYear W20160843902020 @default.
• W2016084390 crossrefType "journal-article" @default.
• W2016084390 hasAuthorship W2016084390A5001211705 @default.
• W2016084390 hasAuthorship W2016084390A5065542731 @default.
• W2016084390 hasAuthorship W2016084390A5077050364 @default.
• W2016084390 hasConcept C104317684 @default.
• W2016084390 hasConcept C119203544 @default.
• W2016084390 hasConcept C129312508 @default.
• W2016084390 hasConcept C130197536 @default.
• W2016084390 hasConcept C143660080 @default.
• W2016084390 hasConcept C145169222 @default.
• W2016084390 hasConcept C153911025 @default.
• W2016084390 hasConcept C156719811 @default.
• W2016084390 hasConcept C17757408 @default.
• W2016084390 hasConcept C178790620 @default.
• W2016084390 hasConcept C185592680 @default.
• W2016084390 hasConcept C2777563447 @default.
• W2016084390 hasConcept C48023723 @default.
• W2016084390 hasConcept C49105822 @default.
• W2016084390 hasConcept C52001828 @default.
• W2016084390 hasConcept C552990157 @default.
• W2016084390 hasConcept C55493867 @default.
• W2016084390 hasConcept C67705224 @default.
• W2016084390 hasConcept C86803240 @default.
• W2016084390 hasConcept C90583042 @default.
• W2016084390 hasConceptScore W2016084390C104317684 @default.
• W2016084390 hasConceptScore W2016084390C119203544 @default.
• W2016084390 hasConceptScore W2016084390C129312508 @default.
• W2016084390 hasConceptScore W2016084390C130197536 @default.
• W2016084390 hasConceptScore W2016084390C143660080 @default.
• W2016084390 hasConceptScore W2016084390C145169222 @default.
• W2016084390 hasConceptScore W2016084390C153911025 @default.
• W2016084390 hasConceptScore W2016084390C156719811 @default.
• W2016084390 hasConceptScore W2016084390C17757408 @default.
• W2016084390 hasConceptScore W2016084390C178790620 @default.
• W2016084390 hasConceptScore W2016084390C185592680 @default.
• W2016084390 hasConceptScore W2016084390C2777563447 @default.
• W2016084390 hasConceptScore W2016084390C48023723 @default.
• W2016084390 hasConceptScore W2016084390C49105822 @default.
• W2016084390 hasConceptScore W2016084390C52001828 @default.
• W2016084390 hasConceptScore W2016084390C552990157 @default.
• W2016084390 hasConceptScore W2016084390C55493867 @default.
• W2016084390 hasConceptScore W2016084390C67705224 @default.
• W2016084390 hasConceptScore W2016084390C86803240 @default.
• W2016084390 hasConceptScore W2016084390C90583042 @default.
• W2016084390 hasIssue "1-2" @default.
• W2016084390 hasLocation W20160843901 @default.
• W2016084390 hasLocation W20160843902 @default.
• W2016084390 hasOpenAccess W2016084390 @default.
• W2016084390 hasPrimaryLocation W20160843901 @default.
• W2016084390 hasRelatedWork W1161402450 @default.
• W2016084390 hasRelatedWork W1623490740 @default.
• W2016084390 hasRelatedWork W2016084390 @default.
• W2016084390 hasRelatedWork W2018126030 @default.
• W2016084390 hasRelatedWork W2042073917 @default.

• next