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• W2016086328 endingPage "156" @default.
• W2016086328 startingPage "146" @default.
• W2016086328 abstract "In spite of recent advance in understanding of the stoichiometry of 22-kDa human GH (22K-hGH) with cell surface hGH receptor (hGHR) and hGH-binding protein (hGH-BP) circulating in human plasma, that of 20-kDa hGH (20K-hGH) is poorly understood. To clarify this, mouse pro-B Ba/F3 cells stably expressing the full-length hGHR (Ba/F3-hGHR) and both recombinant and native hGH-BP were used in this study. Cell proliferation assay revealed that the two hGH isoforms increased Ba/F3-hGHR cells to the same extent in a dose-dependent manner at 0.1 pm–10 nm. However, the self-inhibition observed in 20K-hGH at 5 μm was significantly less than that in 22K-hGH. Furthermore, addition of 1 and 10 nm recombinant hGH-BP caused a slight inhibition in 20K-hGH, but a drastic inhibition in 22K-hGH. Gel filtration chromatography of mixtures of 20K-hGH with recombinant hGH-BP clearly demonstrated that 20K-hGH formed a 1:2 (hGH:hGH-BP) complex efficiently but no detectable 1:1 complex in any conditions. Supporting data were also obtained with native hGH-BP. Computer-aided homology modeling of 20K-hGH has provided speculative data that the conformational change caused by deletion of 15 residues may occur only in the loop between helix 1 and helix 2, resulting in the reduction of its site 1 affinity. In conclusion, 20K-hGH possesses a unique property for forming a 1:2 complex to the same extent as 22K-hGH but has difficulty in forming a 1:1 complex, which might be attributed to the conformational change restricted to its site 1 region." @default.
• W2016086328 created "2016-06-24" @default.
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• W2016086328 date "1998-01-01" @default.
• W2016086328 modified "2023-09-24" @default.
• W2016086328 title "The 20-Kilodalton (kDa) Human Growth Hormone (hGH) Differs from the 22-kDa hGH in the Complex Formation with Cell Surface hGH Receptor and hGH-Binding Protein Circulating in Human Plasma" @default.
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• W2016086328 doi "https://doi.org/10.1210/mend.12.1.0054" @default.
• W2016086328 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9440818" @default.
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