FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016086484> ?p ?o ?g. }

• W2016086484 endingPage "1427" @default.
• W2016086484 startingPage "1425" @default.
• W2016086484 abstract "Just more than a decade ago, a report of a rare human genetic disorder initiated a new spurt of research that profoundly changed our understanding of the glomerular filtration barrier. In that landmark article,1 mutations in the gene encoding the adhesion protein nephrin identified the cause of congenital nephrotic syndrome of the Finnish type. Subsequent studies localized nephrin to the podocyte slit diaphragm, a specialized cell junction that connects neighboring podocytes, the visceral epithelial cells of the glomerulus. These neighboring podocytes elaborate long, regularly spaced, interdigitated foot processes that enwrap the glomerular capillaries and form a 40-nm-wide filtration slit bridged by the slit diaphragm. Further studies suggested that nephrin and associated proteins, in addition to their role as structural components of the slit diaphragm, act as signaling molecules to control podocyte development, cytoskeletal architecture, cell shape and viability, and the function of the glomerular filtration barrier.2–4 Much insight into the signaling function of the slit diaphragm protein complex resulted from the identification of nephrin-interacting proteins,5–9 yet the mechanisms that control ultrastructure and shape of the highly organized podocyte foot process remain poorly understood. This changed with the finding that nephrin binds Src homology 2 (SH2)/SH3 domain-containing Nck adaptor proteins, which in turn seems to control the podocyte cytoskeleton in vivo.10,11 The Nck family of adaptors (mammals carry two related Nck genes, Nck1 and Nck2) functions in coupling phosphotyrosine signals to actin cytoskeletal reorganization. Each of these proteins contain multiple protein interaction domains: three SH3 domains that are known to interact with proline-rich binding partners such as the actin organizers WASP, SPIN90, and dynamin and an SH2 domain that mediates binding of transmembrane receptors and adhesion proteins through phosphotyrosine-dependent protein interactions.12 Nephrin ectodomains recruit the Src family tyrosine kinase Fyn to phosphorylate the nephrin cytoplasmic tail, allowing recruitment of Nck and inducing localized Nck-driven actin polymerization.13 Notably, selective gene deletion of Nck1 and Nck2 from podocytes in transgenic mice results in severe defects in the formation of podocyte processes and in congenital nephrotic syndrome.10 Although the profound changes in cell shape and podocyte morphology in this model mirror the stereotypical reaction of podocytes to injury or damage,14,15 the phenotype is clearly a developmental defect–mice are born without Nck1 or Nck2 in podocytes—and does not clarify the important question of whether slit diaphragm signaling and nephrin-mediated recruitment of Nck are involved in actively regulating podocyte cell shape and maintaining the ultrastructural and functional integrity of the glomerular filtration barrier in vivo. In a remarkable article in this issue of JASN, Jones et al.16 now show that inducible gene deletion of Nck in adult transgenic mice results in podocyte cell shape changes, massive proteinuria, and the development of glomerular scarring. Jones et al. use an inducible transgenic strategy that allows selective deletion of Nck expression in podocytes of adult mice. The authors carefully analyze the ultrastructural changes of podocytes in adult mice lacking Nck expression and definitively demonstrate that Nck1 and Nck2 maintain the shape and integrity of foot processes. This is interesting for a number of reasons. In response to injury, podocytes undergo a dramatic change in morphology—termed foot process effacement—resulting from alterations in cytoskeletal and intercellular junctional architecture. Effacement results from simplification of foot processes, namely loss of foot process shape and retraction, widening, and shortening of the processes of each podocyte. The frequency of filtration slits is also reduced, giving the appearance of a continuous cytoplasmic sheet covering the glomerular basement membrane.15 Effacement is not specific to one disease but rather is synonymous with podocyte injury of many forms, providing a fluid and reversible process that directly correlates with the development of proteinuria both in human disease and in experimental models. Thus, it is interesting that Nck gene deletion and, accordingly, loss of nephrin-dependent Nck recruitment exactly mirror this phenotype. The glomerular morphology of gene-deleted mice undergo the widely known continuum from mild foot process widening to focal areas of fusion and the development of extensive effacement that mirrors minimal-change disease in humans; however, these changes obviously precede the loss of podocytes and the development of focal areas of glomerulosclerosis,16 which is consistent with FSGS in humans, raising the hypothesis that minimal-change disease and FSGS in many cases are two extremes of an ellipse. Nephrin and associated proteins are conserved through evolution and play important roles in patterning of the eye in Drosophila melanogaster or synapse targeting in Caenorhabditis elegans. All of these changes require defined cytoskeletal rearrangements; therefore, it will be important to determine whether nephrin-induced Nck activity is also required in these events. As Jones et al.16 clearly demonstrate, nephrin phosphorylation is maintained in the adult glomerulus supporting the importance of signaling at the slit diaphragm for regulation of podocyte cell shape, cytoskeletal architecture, and cell viability. It is worth noting that these new findings have the same limitations as the original report concerning a developmental role of nephrin-Nck signaling. Although Jones et al. clearly demonstrate that Nck1 and Nck2 are essential for developing and maintaining the complex ultrastructure of podocyte secondary processes, both articles lack direct proof that Nck proteins exclusively act through the nephrin/slit diaphragm axis in vivo. In fact, this has to be addressed in future studies, for instance using knock-in mouse models of nephrin with mutated tyrosine residues. Despite this limitation, Jones et al.16 undoubtedly demonstrate the importance of Nck in controlling the ultrastructure of podocyte foot processes in maintaining the integrity of the glomerular filtration barrier. DISCLOSURES None. I thank members of the Benzing laboratory for helpful discussions and critically reading the manuscript." @default.
• W2016086484 created "2016-06-24" @default.
• W2016086484 creator A5050423319 @default.
• W2016086484 date "2009-07-01" @default.
• W2016086484 modified "2023-10-17" @default.
• W2016086484 title "The Promise of Well-Being" @default.
• W2016086484 cites W1972807210 @default.
• W2016086484 cites W1972845034 @default.
• W2016086484 cites W1974229450 @default.
• W2016086484 cites W2049804001 @default.
• W2016086484 cites W2056363506 @default.
• W2016086484 cites W2059946666 @default.
• W2016086484 cites W2085574022 @default.
• W2016086484 cites W2099365070 @default.
• W2016086484 cites W2121944672 @default.
• W2016086484 cites W2121977177 @default.
• W2016086484 cites W2129416468 @default.
• W2016086484 cites W2132619856 @default.
• W2016086484 cites W2135679847 @default.
• W2016086484 cites W2150611448 @default.
• W2016086484 cites W2164682544 @default.
• W2016086484 cites W4231951653 @default.
• W2016086484 doi "https://doi.org/10.1681/asn.2009040453" @default.
• W2016086484 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19497966" @default.
• W2016086484 hasPublicationYear "2009" @default.
• W2016086484 type Work @default.
• W2016086484 sameAs 2016086484 @default.
• W2016086484 citedByCount "6" @default.
• W2016086484 countsByYear W20160864842013 @default.
• W2016086484 countsByYear W20160864842014 @default.
• W2016086484 countsByYear W20160864842018 @default.
• W2016086484 countsByYear W20160864842020 @default.
• W2016086484 crossrefType "journal-article" @default.
• W2016086484 hasAuthorship W2016086484A5050423319 @default.
• W2016086484 hasBestOaLocation W20160864841 @default.
• W2016086484 hasConcept C108636557 @default.
• W2016086484 hasConcept C142669718 @default.
• W2016086484 hasConcept C1491633281 @default.
• W2016086484 hasConcept C183786373 @default.
• W2016086484 hasConcept C196347352 @default.
• W2016086484 hasConcept C2777146197 @default.
• W2016086484 hasConcept C2779561371 @default.
• W2016086484 hasConcept C2780091579 @default.
• W2016086484 hasConcept C2780819817 @default.
• W2016086484 hasConcept C3018201436 @default.
• W2016086484 hasConcept C54355233 @default.
• W2016086484 hasConcept C62478195 @default.
• W2016086484 hasConcept C86803240 @default.
• W2016086484 hasConcept C95444343 @default.
• W2016086484 hasConceptScore W2016086484C108636557 @default.
• W2016086484 hasConceptScore W2016086484C142669718 @default.
• W2016086484 hasConceptScore W2016086484C1491633281 @default.
• W2016086484 hasConceptScore W2016086484C183786373 @default.
• W2016086484 hasConceptScore W2016086484C196347352 @default.
• W2016086484 hasConceptScore W2016086484C2777146197 @default.
• W2016086484 hasConceptScore W2016086484C2779561371 @default.
• W2016086484 hasConceptScore W2016086484C2780091579 @default.
• W2016086484 hasConceptScore W2016086484C2780819817 @default.
• W2016086484 hasConceptScore W2016086484C3018201436 @default.
• W2016086484 hasConceptScore W2016086484C54355233 @default.
• W2016086484 hasConceptScore W2016086484C62478195 @default.
• W2016086484 hasConceptScore W2016086484C86803240 @default.
• W2016086484 hasConceptScore W2016086484C95444343 @default.
• W2016086484 hasIssue "7" @default.
• W2016086484 hasLocation W20160864841 @default.
• W2016086484 hasLocation W20160864842 @default.
• W2016086484 hasOpenAccess W2016086484 @default.
• W2016086484 hasPrimaryLocation W20160864841 @default.
• W2016086484 hasRelatedWork W1968016907 @default.
• W2016086484 hasRelatedWork W1974229450 @default.
• W2016086484 hasRelatedWork W2009109782 @default.
• W2016086484 hasRelatedWork W2069000231 @default.
• W2016086484 hasRelatedWork W2125087346 @default.
• W2016086484 hasRelatedWork W2150611448 @default.
• W2016086484 hasRelatedWork W2589320596 @default.
• W2016086484 hasRelatedWork W2785421760 @default.
• W2016086484 hasRelatedWork W4210445361 @default.
• W2016086484 hasRelatedWork W4214667068 @default.
• W2016086484 hasVolume "20" @default.
• W2016086484 isParatext "false" @default.
• W2016086484 isRetracted "false" @default.
• W2016086484 magId "2016086484" @default.
• W2016086484 workType "article" @default.