FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016086592> ?p ?o ?g. }

• W2016086592 endingPage "206" @default.
• W2016086592 startingPage "178" @default.
• W2016086592 abstract "This article discusses the application of thermodynamic and related analysis to reaction systems for enzymic or whole cell catalysis, in which there are high proportions of organic liquid, gas, or supercritical fluid. A variety of predictions may be made, especially based on the partitioning of components between the different phases normally present. In many cases, observed behavior can be explained without invoking any direct molecular effects on the biocatalyst. The predictable changes should always be allowed for before seeking explanations for the residual effects, which are often very different from the crude observations. A summary of the general thermodynamics of multiphase systems is presented, and then the main classes of component that distribute between the phases are discussed in turn. Thermodynamic water activity (aw) determines the mass action effects of water on hydrolytic equilibria. It also describes the distribution of water between the various phases that can compete in binding water. Because catalytic activity is very sensitive to the hydration of the enzyme molecules, aw often predicts an unchanging optimum as other aspects of the system are changed. Hence the aw should be measured and/or controlled in these systems, whether the primary aim is to study the effects of water or of other changes. The methods available for measurement and control of aw are discussed. Adverse effects of organic solvents or similar nonpolar species partly reflect their tendency to partition into the relatively polar phase around the biocatalyst, especially when this is dilute aqueous. The well-established log P parameter is a measure of this. But other mechanisms of inactivation can occur: directly through contact of the biocatalyst with the phase interface, or indirectly via hydration changes. In these cases the molecular property log P is probably not the best solvent parameter. In low-water systems the biocatalyst remains in a separate phase even when water-miscible solvents are used. Hence, the categorization of solvents in terms of miscibility becomes less relevant. This accounts for the two peak dependence of catalytic activity on water content in some miscible systems. Differential solvation of reactants and products, as the bulk phase is altered, causes changes in concentration-based equilibrium constants and yields. These changes in solvation may be monitored through partition coefficient or solubility measurements. Reactant solvation can also account for differences in biocatalyst kinetics, whether or not partitioning into a dilute aqueous phase is involved. These predictable effects should be allowed for when studying effects of solvent or similar changes on activity or specificity.(ABSTRACT TRUNCATED AT 400 WORDS)" @default.
• W2016086592 created "2016-06-24" @default.
• W2016086592 creator A5076901096 @default.
• W2016086592 date "1994-03-01" @default.
• W2016086592 modified "2023-10-17" @default.
• W2016086592 title "Thermodynamic predictions for biocatalysis in nonconventional media: Theory, tests, and recommendations for experimental design and analysis" @default.
• W2016086592 cites W1487189791 @default.
• W2016086592 cites W1526437663 @default.
• W2016086592 cites W1544750576 @default.
• W2016086592 cites W1556642547 @default.
• W2016086592 cites W1565644117 @default.
• W2016086592 cites W1582808982 @default.
• W2016086592 cites W1672810509 @default.
• W2016086592 cites W177408800 @default.
• W2016086592 cites W1963559630 @default.
• W2016086592 cites W1965890081 @default.
• W2016086592 cites W1967540987 @default.
• W2016086592 cites W1970429776 @default.
• W2016086592 cites W1972157875 @default.
• W2016086592 cites W1972788243 @default.
• W2016086592 cites W1974472010 @default.
• W2016086592 cites W1974752447 @default.
• W2016086592 cites W1974919027 @default.
• W2016086592 cites W1976317156 @default.
• W2016086592 cites W1977198119 @default.
• W2016086592 cites W1978210865 @default.
• W2016086592 cites W1982392910 @default.
• W2016086592 cites W1982874812 @default.
• W2016086592 cites W1983095441 @default.
• W2016086592 cites W1987373059 @default.
• W2016086592 cites W1992241977 @default.
• W2016086592 cites W1992535099 @default.
• W2016086592 cites W1993364844 @default.
• W2016086592 cites W1995779263 @default.
• W2016086592 cites W1996527996 @default.
• W2016086592 cites W1998728524 @default.
• W2016086592 cites W2003308792 @default.
• W2016086592 cites W2007720050 @default.
• W2016086592 cites W2007853374 @default.
• W2016086592 cites W2008363642 @default.
• W2016086592 cites W2008869811 @default.
• W2016086592 cites W2009513954 @default.
• W2016086592 cites W2010515695 @default.
• W2016086592 cites W2011739595 @default.
• W2016086592 cites W2013800077 @default.
• W2016086592 cites W2015484843 @default.
• W2016086592 cites W2015569421 @default.
• W2016086592 cites W2015878719 @default.
• W2016086592 cites W2018215401 @default.
• W2016086592 cites W2019427047 @default.
• W2016086592 cites W2020340291 @default.
• W2016086592 cites W2021503153 @default.
• W2016086592 cites W2021999454 @default.
• W2016086592 cites W2023303581 @default.
• W2016086592 cites W2029275114 @default.
• W2016086592 cites W2030295708 @default.
• W2016086592 cites W2030795605 @default.
• W2016086592 cites W2031435726 @default.
• W2016086592 cites W2032359150 @default.
• W2016086592 cites W2033442503 @default.
• W2016086592 cites W2035702573 @default.
• W2016086592 cites W2036325181 @default.
• W2016086592 cites W2039339517 @default.
• W2016086592 cites W2042529571 @default.
• W2016086592 cites W2042840613 @default.
• W2016086592 cites W2048078168 @default.
• W2016086592 cites W2048445160 @default.
• W2016086592 cites W2048844013 @default.
• W2016086592 cites W2050383571 @default.
• W2016086592 cites W2051151916 @default.
• W2016086592 cites W2051204691 @default.
• W2016086592 cites W2052923994 @default.
• W2016086592 cites W2054476692 @default.
• W2016086592 cites W2056855390 @default.
• W2016086592 cites W2057489414 @default.
• W2016086592 cites W2060184696 @default.
• W2016086592 cites W2062958450 @default.
• W2016086592 cites W2063776263 @default.
• W2016086592 cites W2064985385 @default.
• W2016086592 cites W2065321595 @default.
• W2016086592 cites W2066725588 @default.
• W2016086592 cites W2070206287 @default.
• W2016086592 cites W2071440610 @default.
• W2016086592 cites W2072696894 @default.
• W2016086592 cites W2073434308 @default.
• W2016086592 cites W2074101649 @default.
• W2016086592 cites W2077110298 @default.
• W2016086592 cites W2078807194 @default.
• W2016086592 cites W2079583693 @default.
• W2016086592 cites W2079626310 @default.
• W2016086592 cites W2079909996 @default.
• W2016086592 cites W2080210800 @default.
• W2016086592 cites W2081619780 @default.
• W2016086592 cites W2084527758 @default.
• W2016086592 cites W2084593913 @default.
• W2016086592 cites W2085451705 @default.
• W2016086592 cites W2086103783 @default.
• W2016086592 cites W2086632131 @default.

• next