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- W2016087254 abstract "Two rapid modifications of a tube assay for the detection of beta-glucuronidase activity (PGUA assay) were evaluated for the identification of Escherichia coli from urine cultures. A microwell and filter paper adaptation of the PGUA assay were tested using 1,234 oxidase-negative, Gram-negative rods isolated from urine on MacConkey agar in clinically significant numbers. There was perfect correlation between both methods and 676 of 797 E. coli isolates were PGUA-positive within 2 h while all of remaining isolates were PGUA-negative (sensitivity = 85%; specificity = 100%). We conclude that either modified format of the PGUA assay provides a useful, inexpensive, and rapid alternative spot test for the definitive identification of E. coli from urine because of the high degree of specificity." @default.
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- W2016087254 date "2001-02-01" @default.
- W2016087254 modified "2023-09-27" @default.
- W2016087254 title "Evaluation of two rapid modifications of the 4-nitrophenyl-β-D-glucopyranosiduronic acid (PGUA) assay for the identification of Escherichia coli from urine" @default.
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- W2016087254 doi "https://doi.org/10.1016/s0732-8893(00)00229-7" @default.
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