Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016087274> ?p ?o ?g. }
- W2016087274 endingPage "794" @default.
- W2016087274 startingPage "783" @default.
- W2016087274 abstract "Abstract The Smt3 (SUMO) protein is conjugated to substrate proteins through a cascade of E1, E2, and E3 enzymes. In budding yeast, the E3 step in sumoylation is largely controlled by Siz1p and Siz2p. Analysis of Siz− cells shows that SUMO E3 is required for minichromosome segregation and thus has a positive role in maintaining the fidelity of mitotic transmission of genetic information. Sumoylation of the carboxy-terminus of Top2p, a known SUMO target, is mediated by Siz1p and Siz2p both in vivo and in vitro. Sumoylation in vitro reveals that Top2p is an extremely potent substrate for Smt3p conjugation and that chromatin-bound Top2p can still be sumoylated, unlike many other SUMO substrates. By combining mutations in the TOP2 sumoylation sites and the SIZ1 and SIZ2 genes we demonstrate that the minichromosome segregation defect and dicentric minichromosome stabilization, both characteristic for Smt3p–E3-deficient cells, are mediated by the lack of Top2p sumoylation in these cells. A role for Smt3p-modification as a signal for Top2p targeting to pericentromeric regions was suggested by an analysis of Top2p–Smt3p fusion. We propose a model for the positive control of the centromeric pool of Top2p, required for high segregation fidelity, by Smt3p modification." @default.
- W2016087274 created "2016-06-24" @default.
- W2016087274 creator A5019618575 @default.
- W2016087274 creator A5024531723 @default.
- W2016087274 creator A5049230932 @default.
- W2016087274 creator A5074934459 @default.
- W2016087274 date "2006-02-01" @default.
- W2016087274 modified "2023-10-12" @default.
- W2016087274 title "<i>SIZ1/SIZ2</i> Control of Chromosome Transmission Fidelity Is Mediated by the Sumoylation of Topoisomerase II" @default.
- W2016087274 cites W1515980537 @default.
- W2016087274 cites W1600888450 @default.
- W2016087274 cites W1640183712 @default.
- W2016087274 cites W1666755687 @default.
- W2016087274 cites W1780544763 @default.
- W2016087274 cites W1832847580 @default.
- W2016087274 cites W1951655399 @default.
- W2016087274 cites W1966072209 @default.
- W2016087274 cites W1967390330 @default.
- W2016087274 cites W1970061483 @default.
- W2016087274 cites W1971358793 @default.
- W2016087274 cites W1972391154 @default.
- W2016087274 cites W1974875206 @default.
- W2016087274 cites W1976251488 @default.
- W2016087274 cites W1985313012 @default.
- W2016087274 cites W1991326375 @default.
- W2016087274 cites W1996047595 @default.
- W2016087274 cites W1997161800 @default.
- W2016087274 cites W2001029086 @default.
- W2016087274 cites W2002516756 @default.
- W2016087274 cites W2006247396 @default.
- W2016087274 cites W2006483060 @default.
- W2016087274 cites W2012704893 @default.
- W2016087274 cites W2012734297 @default.
- W2016087274 cites W2024191478 @default.
- W2016087274 cites W2024935012 @default.
- W2016087274 cites W2028638629 @default.
- W2016087274 cites W2029189334 @default.
- W2016087274 cites W2032360849 @default.
- W2016087274 cites W2034217568 @default.
- W2016087274 cites W2040825545 @default.
- W2016087274 cites W2046037983 @default.
- W2016087274 cites W2051094352 @default.
- W2016087274 cites W2053060343 @default.
- W2016087274 cites W2057530681 @default.
- W2016087274 cites W2067371211 @default.
- W2016087274 cites W2070163476 @default.
- W2016087274 cites W2081446916 @default.
- W2016087274 cites W2083312621 @default.
- W2016087274 cites W2090662981 @default.
- W2016087274 cites W2091488974 @default.
- W2016087274 cites W2091602394 @default.
- W2016087274 cites W2099063441 @default.
- W2016087274 cites W2110548948 @default.
- W2016087274 cites W2112441454 @default.
- W2016087274 cites W2116251465 @default.
- W2016087274 cites W2119301405 @default.
- W2016087274 cites W2121428233 @default.
- W2016087274 cites W2123256728 @default.
- W2016087274 cites W2129581798 @default.
- W2016087274 cites W2131424666 @default.
- W2016087274 cites W2132600317 @default.
- W2016087274 cites W2134954412 @default.
- W2016087274 cites W2137507610 @default.
- W2016087274 cites W2139114666 @default.
- W2016087274 cites W2139582206 @default.
- W2016087274 cites W2139633758 @default.
- W2016087274 cites W2145688254 @default.
- W2016087274 cites W2152826893 @default.
- W2016087274 cites W2154805387 @default.
- W2016087274 cites W2168796310 @default.
- W2016087274 cites W2169333620 @default.
- W2016087274 cites W2169805130 @default.
- W2016087274 cites W2412667103 @default.
- W2016087274 cites W2791163698 @default.
- W2016087274 doi "https://doi.org/10.1534/genetics.105.047167" @default.
- W2016087274 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1456244" @default.
- W2016087274 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16204216" @default.
- W2016087274 hasPublicationYear "2006" @default.
- W2016087274 type Work @default.
- W2016087274 sameAs 2016087274 @default.
- W2016087274 citedByCount "84" @default.
- W2016087274 countsByYear W20160872742012 @default.
- W2016087274 countsByYear W20160872742013 @default.
- W2016087274 countsByYear W20160872742014 @default.
- W2016087274 countsByYear W20160872742015 @default.
- W2016087274 countsByYear W20160872742016 @default.
- W2016087274 countsByYear W20160872742017 @default.
- W2016087274 countsByYear W20160872742018 @default.
- W2016087274 countsByYear W20160872742019 @default.
- W2016087274 countsByYear W20160872742020 @default.
- W2016087274 countsByYear W20160872742021 @default.
- W2016087274 countsByYear W20160872742022 @default.
- W2016087274 crossrefType "journal-article" @default.
- W2016087274 hasAuthorship W2016087274A5019618575 @default.
- W2016087274 hasAuthorship W2016087274A5024531723 @default.
- W2016087274 hasAuthorship W2016087274A5049230932 @default.
- W2016087274 hasAuthorship W2016087274A5074934459 @default.
- W2016087274 hasBestOaLocation W20160872741 @default.