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- W2016088560 abstract "Motile cilia are plasma membrane projections that contain a central core of microtubules whose movement relative to each other contributes to ciliary beating. The left-right body patterning of developing embryos is thought to result from cilia-dependent differential distribution of morphogens. Knockout of inositol 1,3,4,5,6-pentakisphosphate (IP 5 ) 2-kinase (Ipk1), the enzyme that synthesizes IP 6 , in zebrafish embryos results in defective left-right positioning of organs, although the mechanism involved is unknown. Sarmah et al . extended this work and knocked down expression of ipk1 in zebrafish by injecting embryos with an antisense morpholino oligonucleotide (MO) targeted to ipk1 mRNA ( ipk1 MO1 embryos). The Kupffer’s vesicle (KV) is the organ that initiates left-right organ placement in the zebrafish. The authors found that whereas KV cilia from wild-type embryos beat with a regular frequency in a counterclockwise direction, those from ipk1 MO1 embryos vibrated without a defined direction of motion. Furthermore, immunocytochemical analyses revealed that cilia in ipk1 MO1 embryos were shorter than those in wild-type embryos. Although the ectopic expression in ipk1 MO1 embryos of Ipk1 resulted in the rescue of ciliary length and motion, that of a catalytically inactive mutant of Ipk1 did not. Fluorescence microscopy showed that Ipk1 was localized to centrosomes in the basal bodies of cilia in wild-type embryos. Transmission electron microscopy demonstrated that the organization of microtubules in the cilia from ipk1 MO1 embryos was similar to that in wild-type embryos. However, the anterograde microtubule-dependent movement of melanosomes (pigmented vesicles), which is mediated by kinesin proteins, was much slower in ipk1 MO1 cilia than in wild-type cilia. Together these data implicate Ipk1, and potentially its product IP 6 , in the maintenance of cilia length and the regulation of ciliary motion. B. Sarmah, V. P. Winfrey, G. E. Olson, B. Appel, S. R. Wente, A role for the inositol kinase Ipk1 in ciliary beating and length maintenance. Proc. Natl. Acad. Sci. U.S.A. 104 , 19843-19848 (2007). [Abstract][Full Text]" @default.
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- W2016088560 date "2007-12-11" @default.
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- W2016088560 title "IP6 Got Rhythm?" @default.
- W2016088560 doi "https://doi.org/10.1126/stke.4172007tw455" @default.
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