Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016088891> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2016088891 endingPage "4500" @default.
- W2016088891 startingPage "4496" @default.
- W2016088891 abstract "Several lines of evidence are presented to allow us to conclude that tobacco mosaic virus (TMV) RNA enters the chloroplast in vivo. Chloroplasts were prepared from either directly inoculated or systemically infected leaves of tobacco plants inoculated with one of several strains of the virus and from uninfected control plants. Intact chloroplasts were isolated on Percoll gradients and treated with pancreatic RNase and thermolysin to destroy potential TMV virions and RNA on the outside or bound to their surfaces. Northern blot analysis of RNA extracted from these chloroplasts demonstrated that full-length TMV RNA was present within the chloroplasts prepared from both directly inoculated and systemically invaded leaves. Only genomic length, but not subgenomic length, RNA was found in the chloroplast extracts, indicating a selectivity of the transport of the viral RNA into the chloroplast. A temperature-sensitive TMV mutant (Ts 38), in which no virions are formed at 35 degrees C, was used to demonstrate that at that restrictive temperature viral RNA is detected in the chloroplast, indicating that free viral RNA can enter the chloroplast rather than intact virions. To our knowledge, the transport of a foreign RNA species into chloroplasts has not been reported previously." @default.
- W2016088891 created "2016-06-24" @default.
- W2016088891 creator A5036644505 @default.
- W2016088891 creator A5080674738 @default.
- W2016088891 date "1989-06-01" @default.
- W2016088891 modified "2023-09-27" @default.
- W2016088891 title "Tobacco mosaic virus RNA enters chloroplasts <i>in vivo</i>" @default.
- W2016088891 cites W1510003654 @default.
- W2016088891 cites W1546538601 @default.
- W2016088891 cites W1967839572 @default.
- W2016088891 cites W1980279189 @default.
- W2016088891 cites W1990016313 @default.
- W2016088891 cites W1994037409 @default.
- W2016088891 cites W1999910604 @default.
- W2016088891 cites W2005394798 @default.
- W2016088891 cites W2013398799 @default.
- W2016088891 cites W2050913265 @default.
- W2016088891 cites W2057323302 @default.
- W2016088891 cites W2065507478 @default.
- W2016088891 cites W2073888029 @default.
- W2016088891 cites W2082831376 @default.
- W2016088891 cites W2087461594 @default.
- W2016088891 cites W2089144534 @default.
- W2016088891 cites W2139407026 @default.
- W2016088891 cites W2166064969 @default.
- W2016088891 doi "https://doi.org/10.1073/pnas.86.12.4496" @default.
- W2016088891 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/287297" @default.
- W2016088891 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16578844" @default.
- W2016088891 hasPublicationYear "1989" @default.
- W2016088891 type Work @default.
- W2016088891 sameAs 2016088891 @default.
- W2016088891 citedByCount "38" @default.
- W2016088891 countsByYear W20160888912012 @default.
- W2016088891 countsByYear W20160888912013 @default.
- W2016088891 countsByYear W20160888912014 @default.
- W2016088891 countsByYear W20160888912015 @default.
- W2016088891 countsByYear W20160888912016 @default.
- W2016088891 countsByYear W20160888912017 @default.
- W2016088891 countsByYear W20160888912018 @default.
- W2016088891 crossrefType "journal-article" @default.
- W2016088891 hasAuthorship W2016088891A5036644505 @default.
- W2016088891 hasAuthorship W2016088891A5080674738 @default.
- W2016088891 hasBestOaLocation W20160888912 @default.
- W2016088891 hasConcept C104317684 @default.
- W2016088891 hasConcept C144707691 @default.
- W2016088891 hasConcept C153911025 @default.
- W2016088891 hasConcept C159047783 @default.
- W2016088891 hasConcept C189819185 @default.
- W2016088891 hasConcept C2522874641 @default.
- W2016088891 hasConcept C2778786252 @default.
- W2016088891 hasConcept C2778976237 @default.
- W2016088891 hasConcept C55493867 @default.
- W2016088891 hasConcept C67705224 @default.
- W2016088891 hasConcept C69305403 @default.
- W2016088891 hasConcept C86803240 @default.
- W2016088891 hasConceptScore W2016088891C104317684 @default.
- W2016088891 hasConceptScore W2016088891C144707691 @default.
- W2016088891 hasConceptScore W2016088891C153911025 @default.
- W2016088891 hasConceptScore W2016088891C159047783 @default.
- W2016088891 hasConceptScore W2016088891C189819185 @default.
- W2016088891 hasConceptScore W2016088891C2522874641 @default.
- W2016088891 hasConceptScore W2016088891C2778786252 @default.
- W2016088891 hasConceptScore W2016088891C2778976237 @default.
- W2016088891 hasConceptScore W2016088891C55493867 @default.
- W2016088891 hasConceptScore W2016088891C67705224 @default.
- W2016088891 hasConceptScore W2016088891C69305403 @default.
- W2016088891 hasConceptScore W2016088891C86803240 @default.
- W2016088891 hasIssue "12" @default.
- W2016088891 hasLocation W20160888911 @default.
- W2016088891 hasLocation W20160888912 @default.
- W2016088891 hasLocation W20160888913 @default.
- W2016088891 hasLocation W20160888914 @default.
- W2016088891 hasOpenAccess W2016088891 @default.
- W2016088891 hasPrimaryLocation W20160888911 @default.
- W2016088891 hasRelatedWork W172848956 @default.
- W2016088891 hasRelatedWork W1924104928 @default.
- W2016088891 hasRelatedWork W1975209705 @default.
- W2016088891 hasRelatedWork W1987346219 @default.
- W2016088891 hasRelatedWork W2016088891 @default.
- W2016088891 hasRelatedWork W2036598563 @default.
- W2016088891 hasRelatedWork W2069612994 @default.
- W2016088891 hasRelatedWork W2082775471 @default.
- W2016088891 hasRelatedWork W2083020885 @default.
- W2016088891 hasRelatedWork W2740730431 @default.
- W2016088891 hasVolume "86" @default.
- W2016088891 isParatext "false" @default.
- W2016088891 isRetracted "false" @default.
- W2016088891 magId "2016088891" @default.
- W2016088891 workType "article" @default.