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- W2016093676 abstract "To determine the pathway for 20:5 (n−3) synthesis in the marine diatom, Phaeodactylum tricornutum, pulse-labelling and chase experiments with labelled fatty acids were carried out. [14C] 20:5 (n−3) was synthesized from [1-14C] 18:1 (n−9), [1-14C] 18:2 (n−6), [1-14C] 18:3 (n−3), [2-14C] 20:3 (n−6) or [1-14C] 20:4 (n−6). The rates of [14C] 20:5 (n−3) synthesis from [14C] fatty acids were [1-14C] 20:4 (n−6)>[2-14C] 20:3 (n−6)>[1-14C] 18:2 (n−6)>[1-14C] 18:3 n−3)>[1-14C] 18:1 (n−9). When pulse-labelled with [1-14C] 18:1 (n−9) to synthesize 20:5 (n−3), the intermediates such as [14C] 18:2 (n−6), [14C] 18:4 (n−3), [14C] 20:4 (n−6) and [14C] 20:4 (n−3) were accumulated during the chase. When pulse-labelled with [1-14C] 18:2 (n−6) instead, both [14C] 18:3 (n−6) and [14C] 18:3 (n−3) were accumulated during the chase. Similarly, [14C] 20:3 (n−3) was accumulated in pulse-chase with [1-14C] 18:3 (n−3) and [14C] 20:4 (n−6) in pulse-chase with [2-14C] 20:3 (n−6). Incorporation of [1-14C] 18:1 (n−9) into [14C] 20:5 (n−3) was inhibited in the presence of cold fatty acids such as 18:1 (n−9), 18:2 (n−6), 18:3 (n−3), 18:3 (n−6), 20:3 (n−3), 20:3 (n−6), 20:4 (n−6) or 20:5 (n−3), but not inhibited in the presence of 20:1 (n−9) or 20:2 (n−6). From these results, it is concluded that 20:5 (n−3) is able to be synthesized from 18:1 (n−9) in P. tricornutum by a network pathway which contains four routes from 18:2 (n−6) to 20:5 (n−3); two routes pass through (n−3)-fatty acids and one route through (n−6)-fatty acids as intermediates. The other route passes through both (n−3)- and (n−6)-fatty acids as intermediates." @default.
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- W2016093676 title "Biosynthesis of polyunsaturated fatty acids in the marine diatom, Phaeodactylum tricornutum" @default.
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- W2016093676 doi "https://doi.org/10.1016/s0031-9422(00)94817-9" @default.
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