FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016096656> ?p ?o ?g. }

• W2016096656 endingPage "647" @default.
• W2016096656 startingPage "646" @default.
• W2016096656 abstract "Testicular sperm extraction in patients with gonadal failure may be very difficult, and the method used to retrieve sperm may necessitate multiple sampling (1Tournaye H. Verheyen G. Nagy Z.P. Ubaldi F. Goossens A. Silber S. et al.Are there any predictive factors for successful testicular sperm recovery in azoospermic patients?.Hum Reprod. 1997; 12: 80-86Crossref PubMed Scopus (357) Google Scholar, 2Hauser R. Botchon A. Amit A. Yosef D.B. Gamzu R. Paz G. et al.Multiple testicular sampling in non-obstructive azoospermia—is it necessary?.Hum Reprod. 1998; 13: 3081-3085Crossref PubMed Scopus (119) Google Scholar, 3Ron El R. Struss S. Friedler S. Friedler S. Strassburger D. Komarovsky D. et al.Serial sonography and color flow Doppler imaging following testicular and epididymal sperm extraction.Hum Reprod. 1998; 13: 3390-3393Crossref PubMed Scopus (66) Google Scholar, 4Amer M. El Haggar S. Moustafa T. Abd El Naser T. Zohdy W. Testicular sperm extraction impact of testicular histology on outcome, number of biopsies to be performed and optimal time for repetition.Hum Reprod. 1999; 14: 3030-3034Crossref PubMed Scopus (93) Google Scholar). Microdissection techniques may offer the best chance of retrieval in patients with minimal complications (5Schlegel P.N. Testicular sperm extraction microdissection improves sperm yield with minimal tissue excision.Hum Reprod. 1999; 14: 131-135Crossref PubMed Scopus (600) Google Scholar, 6Amer M. Atteyah A. Hany R. Zohdy W. Prospective comparative study between microsurgical and conventional TESE in non-obstructive azoospermia follow up by serial ultrasound examinations.Hum Reprod. 2000; 15: 653-656Crossref PubMed Scopus (165) Google Scholar). However, it may take some time to extract and collect sufficient normal motile testicular sperm for injection of all available oocytes. Delayed injection is associated with low fertilization rate and poor embryo quality after ICSI (7Veek L.L. The morphological assessment of human oocytes and early conception.in: Keel B.A. Webster B.W. Laboratory diagnosis and treatment of infertility. CRC Press, Boca Raton (FL)1990: 353Google Scholar, 8Xia P. Intracytoplasmic sperm injection correlation of oocyte grade based on polar body, perivitelline space and cytoplasmic inclusions with fertilization rate and embryo quality.Hum Reprod. 1997; 12: 1750-1755Crossref PubMed Scopus (210) Google Scholar, 9Ebner T. Moser M. Yaman C. Feichtinger O. Hartl J. Tews G. Elective transfer of embryos selected on the basis of first polar body morphology is associated with increased rates of implantation and pregnancy.Fertil Steril. 1999; 72: 599-603Abstract Full Text Full Text PDF PubMed Scopus (88) Google Scholar). We sought to establish clinical and noninvasive laboratory variables that assist in selection of the appropriate surgical sperm retrieval technique, determination of correct timing, and improvement of sperm recovery rate in patients with nonobstructive azoospermia. This retrospective study included 52 patients with nonobstructive azoospermia. The patients underwent history; clinical examination; FSH estimation; and cytogenetic studies, including karyotyping and multiplex polymerase chain reaction to detect Y-chromosome microdeletions. May-Grünwald-Giemsa (MGG) (10Amer M. Abd El Naser T. El Haggar S. Mostafa T. Abd El Malak G. Zohdy W. May-Grünwald-Giemsa stain for detection of spermatogenic cells in the ejaculate a simple predictive parameter for successful testicular sperm retrieval.Hum Reprod. 2001; 16: 1427-1432Crossref PubMed Scopus (26) Google Scholar) and nuclear-fast red-picroindigocarmine staining were used to identify spermatogenic cells and spermatozoa in semen prior to surgery (11Hendin B.N. Patel B. Levin H.S. Thomas A.J. Agarwal A. Identification of spermatozoa and round spermatids in the ejaculates of men with spermatogenic failure.Urology. 1998; 51: 816-819Abstract Full Text Full Text PDF PubMed Scopus (9) Google Scholar). Testicular pathology was quantitatively assessed by using the Johnsen score (12Johnsen S.J. Testicular biopsy score count a method for registration of spermatogenesis in human testis normal values and results in 335 hypogonadal males.Hormones. 1970; 1: 2-25Crossref PubMed Scopus (1206) Google Scholar), in which tubular sections were scored individually and a mean value was calculated for the whole tissue. Late spermatid score was obtained by counting the number of late spermatids in 20 tubules (round sections) and calculating a mean value. Patients were counseled about the success of testicular sperm extraction on the basis of the above variables. Testicular sperm extraction was done by performing microdissection in one side and conventional open surgical methods in the contralateral side, as described elsewhere (7Veek L.L. The morphological assessment of human oocytes and early conception.in: Keel B.A. Webster B.W. Laboratory diagnosis and treatment of infertility. CRC Press, Boca Raton (FL)1990: 353Google Scholar, 12Johnsen S.J. Testicular biopsy score count a method for registration of spermatogenesis in human testis normal values and results in 335 hypogonadal males.Hormones. 1970; 1: 2-25Crossref PubMed Scopus (1206) Google Scholar). Simultaneous testicular sperm extraction and ICSI, or diagnostic biopsy and freezing, were offered to the patients. The patients were extensively counseled about the risks of each choice. Nine patients had clinical varicocele (six left and three bilateral), two had a history of epididymoorchitis, three had received chemotherapy for Hodgkin’s lymphoma, and four had a history of undescended testis (three unilateral and one bilateral). Sixteen patients (30.8%) had had previous testicular biopsy. Karyotyping and analysis for Y-chromosome microdeletions were done in 50 of 52. Five patients had 47,XXY (Klinefelter) karyotype; of those, two had the mosaic pattern 46,XY/47,XXY (mosaic Klinefelter). Three patients had a 46,XY karyotype with inversion Y,(q11.21, q11.22). Two patients had 46,XY karyotype and pericentric inversion 9, which is considered a normal polymorphism. Microdeletions of the Y chromosome were observed in 4 of 50 patients (8%). Sperm were recovered from 31 of 52 men (59.6%). According to difficulty encountered during testicular sperm extraction, patients were classified into two groups. In group A (34 patients), testicular sperm extraction was difficult and necessitated multiple bilateral microsurgical and conventional sampling techniques (317 samples); sperm was recovered in 13 of these 34 patients (38.2%). In 5 of the 13 patients, more than 12 hours were needed for sperm collection. Erythrocyte lysing buffer (13Nagy Z.P. Devroey P. Verheyen G. Van Steirteghem A.C. Tournaye H. An improved treatment procedure for testicular biopsy specimens offers more efficient sperm recovery case series.Fertil Steril. 1997; 68: 376-379Abstract Full Text PDF PubMed Scopus (54) Google Scholar) increased the sperm recovery rate from 3 of 34 (8.8%) to 5 of 34 (14.7%) patients undergoing microsurgical techniques and from 7 of 34 (20.6%) to 12 of 34 (35.2%) patients undergoing conventional techniques (P>.05). After application of erythrocyte lysing buffer, spermatozoa were retrieved in 12 of 34 (35.2%) patients undergoing conventional techniques and 5 of 34 (14.7%) patients undergoing microsurgical techniques (P>.05). In group B (18 patients), one or two small microsurgical samples (total, 25 samples) were sufficient to retrieve motile sperm in all patients (sperm recovery rate, 100%). The difference in sperm recovery rate between the groups A and B could not be attributed to a specific histopathologic pattern, FSH level, testicular volume, late spermatid, or Johnsen scores. However, difficult sperm retrieval was generally observed in older patients and those with infertility of long duration (Table 1). Spermatocytes and spermatids were identified by MGG stain in most patients in group B (17 of 18 [94.4%]) and more than one third of patients in group A (13 of 34 [38.2%]) (P<.05).TABLE 1Characteristics of the study groups.CharacteristicGroup AGroup BP valueAgeaMann-Whitney t-test.39.4 ± 7.9532.75 ± 6.76<.05Duration of infertilityaMann-Whitney t-test.9.8 ± 6.15.2 ± 3.8<.05FSH level (mIU/mL)aMann-Whitney t-test.17.2 ± 14.118.4 ± 9.8NSTesticular VolumeaMann-Whitney t-test.9.9 ± 3.910.9 ± 3.7NSJohnsen scoreaMann-Whitney t-test.2.4 ± 1.383.28 ± 1.93NSLate spermatid scoreaMann-Whitney t-test.0.21 ± 0.691.0 ± 1.73NSOverall pregnancy rate (%)bFisher exact test.2/13 (15.4)5/18 (27.8)NSNote: Values are means (±SD) NS = not significant.Amer. Predicting difficult testicular sperm retrieval. Fertil Steril 2002.a Mann-Whitney t-test.b Fisher exact test. Open table in a new tab Note: Values are means (±SD) NS = not significant. Amer. Predicting difficult testicular sperm retrieval. Fertil Steril 2002. Spermatocytes without spermatids were more commonly seen in group A (7 of 34 patients [20.5%]) than in group B (1 of 18 patients [5.5%]). Moreover, 20 of 31 (64.5%) patients in whom testicular sperm extraction was successful had round spermatids in their ejaculate. Seventeen of these patients had easy retrieval (group B) and 3 had difficult retrieval (group A) (P<.05). In contrast to MGG stain, nuclear-fast red-picroindigocarmine staining was of no predictive value; this technique detected spermatozoa in only 2 patients in group B. Under optical magnification, seminiferous tubules were found to be homogeneous in 27 patients (79.4%) in group A, from which spermatozoa were retrieved in 2 patients (7.4%). In 7 patients (20.6%) the tubules appeared to be heterogeneous (tubules of small and large diameters); spermatozoa were retrieved from 3 (42.9%) of these patients. In group B, the seminiferous tubules appeared heterogeneous in 16 (88.9%) patients and homogeneous in 2 (11.1%) patients; testicular spermatozoa were successfully retrieved from all patients by using one or two microsurgical samples (P<.05). The overall pregnancy rates were 5 of 18 (27.8%) patients in group B and 2 of 13 (15.4%) patients in group A (P>.05) (Table 1). Testicular sperm extraction in patients with spermatogenic failure is easier at younger ages, when spermatids are detected in the ejaculate, and when testicular parenchyma is heterogeneous on microscopy. Thus, early sperm retrieval and cryopreservation can be advised to avoid possible age-related vascular and parenchymal deterioration. Multiple large conventional biopsies are suggested only when testicular tissues show a homogeneous pattern under optical magnification during microdissection testicular sperm extraction, especially in older men, and if semen smears show only primary spermatocytes or no spermatogenic cells. Finally, in patients with suspected difficult sperm retrieval, open surgical sperm retrieval is better started at least 8 hours before ovum pick-up if ICSI is scheduled on the same day to minimize the risk of in vitro postmaturity oocyte damage. Alternatively, open diagnostic biopsy and sperm cryopreservation can be arranged before stimulation of the female partner is started." @default.
• W2016096656 created "2016-06-24" @default.
• W2016096656 creator A5007642675 @default.
• W2016096656 creator A5012642269 @default.
• W2016096656 creator A5039506273 @default.
• W2016096656 creator A5042566110 @default.
• W2016096656 creator A5050273149 @default.
• W2016096656 creator A5072252517 @default.
• W2016096656 date "2002-09-01" @default.
• W2016096656 modified "2023-09-26" @default.
• W2016096656 title "Preoperative and intraoperative factors that predict difficult testicular sperm retrieval in patients with nonobstructive azoospermia" @default.
• W2016096656 cites W1998765170 @default.
• W2016096656 cites W2024542541 @default.
• W2016096656 cites W2027274170 @default.
• W2016096656 cites W2032122214 @default.
• W2016096656 cites W2042447016 @default.
• W2016096656 cites W2102817601 @default.
• W2016096656 cites W2112393154 @default.
• W2016096656 cites W2129220528 @default.
• W2016096656 cites W2131670695 @default.
• W2016096656 cites W2143197752 @default.
• W2016096656 cites W2170081721 @default.
• W2016096656 doi "https://doi.org/10.1016/s0015-0282(02)03273-9" @default.
• W2016096656 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12215352" @default.
• W2016096656 hasPublicationYear "2002" @default.
• W2016096656 type Work @default.
• W2016096656 sameAs 2016096656 @default.
• W2016096656 citedByCount "9" @default.
• W2016096656 countsByYear W20160966562020 @default.
• W2016096656 countsByYear W20160966562021 @default.
• W2016096656 countsByYear W20160966562022 @default.
• W2016096656 crossrefType "journal-article" @default.
• W2016096656 hasAuthorship W2016096656A5007642675 @default.
• W2016096656 hasAuthorship W2016096656A5012642269 @default.
• W2016096656 hasAuthorship W2016096656A5039506273 @default.
• W2016096656 hasAuthorship W2016096656A5042566110 @default.
• W2016096656 hasAuthorship W2016096656A5050273149 @default.
• W2016096656 hasAuthorship W2016096656A5072252517 @default.
• W2016096656 hasBestOaLocation W20160966561 @default.
• W2016096656 hasConcept C126894567 @default.
• W2016096656 hasConcept C2777420699 @default.
• W2016096656 hasConcept C2777688143 @default.
• W2016096656 hasConcept C2779234561 @default.
• W2016096656 hasConcept C2910159267 @default.
• W2016096656 hasConcept C54355233 @default.
• W2016096656 hasConcept C71924100 @default.
• W2016096656 hasConcept C86803240 @default.
• W2016096656 hasConceptScore W2016096656C126894567 @default.
• W2016096656 hasConceptScore W2016096656C2777420699 @default.
• W2016096656 hasConceptScore W2016096656C2777688143 @default.
• W2016096656 hasConceptScore W2016096656C2779234561 @default.
• W2016096656 hasConceptScore W2016096656C2910159267 @default.
• W2016096656 hasConceptScore W2016096656C54355233 @default.
• W2016096656 hasConceptScore W2016096656C71924100 @default.
• W2016096656 hasConceptScore W2016096656C86803240 @default.
• W2016096656 hasIssue "3" @default.
• W2016096656 hasLocation W20160966561 @default.
• W2016096656 hasLocation W20160966562 @default.
• W2016096656 hasOpenAccess W2016096656 @default.
• W2016096656 hasPrimaryLocation W20160966561 @default.
• W2016096656 hasRelatedWork W1984277315 @default.
• W2016096656 hasRelatedWork W1994036839 @default.
• W2016096656 hasRelatedWork W2053514162 @default.
• W2016096656 hasRelatedWork W2107257800 @default.
• W2016096656 hasRelatedWork W2129220528 @default.
• W2016096656 hasRelatedWork W2605759983 @default.
• W2016096656 hasRelatedWork W2805195250 @default.
• W2016096656 hasRelatedWork W2811475844 @default.
• W2016096656 hasRelatedWork W3141096188 @default.
• W2016096656 hasRelatedWork W1785436556 @default.
• W2016096656 hasVolume "78" @default.
• W2016096656 isParatext "false" @default.
• W2016096656 isRetracted "false" @default.
• W2016096656 magId "2016096656" @default.
• W2016096656 workType "article" @default.