FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016097276> ?p ?o ?g. }

• W2016097276 endingPage "342" @default.
• W2016097276 startingPage "332" @default.
• W2016097276 abstract "Enzyme INtr is the first protein in the nitrogen phosphotransferase pathway. Using an array of biochemical and biophysical tools, we characterized the protein, compared its properties to that of EI of the carbohydrate PTS and, in addition, examined the effect of substitution of all nonexchangeable protons by deuterium (perdeuteration) on the properties of EINtr. Notably, we find that the catalytic function (autophosphorylation and phosphotransfer to NPr) remains unperturbed while its stability is modulated by deuteration. In particular, the deuterated form exhibits a reduction of approximately 4 °C in thermal stability, enhanced oligomerization propensity, as well as increased sensitivity to proteolysis in vitro. We investigated tertiary, secondary, and local structural changes, both in the absence and presence of PEP, using near- and far-UV circular dichroism and Trp fluorescence spectroscopy. Our data demonstrate that the aromatic residues are particularly sensitive probes for detecting effects of deuteration with an enhanced quantum yield upon PEP binding and apparent decreases in tertiary contacts for Tyr and Trp side chains. Trp mutagenesis studies showed that the region around Trp522 responds to binding of both PEP and NPr. The significance of these results in the context of structural analysis of EINtr are evaluated." @default.
• W2016097276 created "2016-06-24" @default.
• W2016097276 creator A5008234020 @default.
• W2016097276 creator A5030685879 @default.
• W2016097276 creator A5032243491 @default.
• W2016097276 creator A5056148684 @default.
• W2016097276 creator A5073264020 @default.
• W2016097276 creator A5076520162 @default.
• W2016097276 creator A5081301991 @default.
• W2016097276 date "2011-03-01" @default.
• W2016097276 modified "2023-09-27" @default.
• W2016097276 title "Deuteration of Escherichia coli Enzyme INtr alters its stability" @default.
• W2016097276 cites W1517629137 @default.
• W2016097276 cites W1584817845 @default.
• W2016097276 cites W1958844676 @default.
• W2016097276 cites W1967817108 @default.
• W2016097276 cites W1968686371 @default.
• W2016097276 cites W1984698646 @default.
• W2016097276 cites W1985077671 @default.
• W2016097276 cites W1996512671 @default.
• W2016097276 cites W2003792985 @default.
• W2016097276 cites W2012574818 @default.
• W2016097276 cites W2016768742 @default.
• W2016097276 cites W2019614916 @default.
• W2016097276 cites W2020844936 @default.
• W2016097276 cites W2024218839 @default.
• W2016097276 cites W2036386995 @default.
• W2016097276 cites W2045318395 @default.
• W2016097276 cites W2056731101 @default.
• W2016097276 cites W2061860586 @default.
• W2016097276 cites W2065492641 @default.
• W2016097276 cites W2066916400 @default.
• W2016097276 cites W2067465236 @default.
• W2016097276 cites W2069902442 @default.
• W2016097276 cites W2073931354 @default.
• W2016097276 cites W2076183099 @default.
• W2016097276 cites W2076232012 @default.
• W2016097276 cites W2086053317 @default.
• W2016097276 cites W2088003156 @default.
• W2016097276 cites W2094594614 @default.
• W2016097276 cites W2105013618 @default.
• W2016097276 cites W2111236685 @default.
• W2016097276 cites W2125269987 @default.
• W2016097276 cites W2140520515 @default.
• W2016097276 cites W2146220380 @default.
• W2016097276 cites W2162279862 @default.
• W2016097276 cites W2162691496 @default.
• W2016097276 cites W2166374078 @default.
• W2016097276 cites W2342321246 @default.
• W2016097276 cites W335769971 @default.
• W2016097276 doi "https://doi.org/10.1016/j.abb.2010.12.022" @default.
• W2016097276 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3058872" @default.
• W2016097276 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21185804" @default.
• W2016097276 hasPublicationYear "2011" @default.
• W2016097276 type Work @default.
• W2016097276 sameAs 2016097276 @default.
• W2016097276 citedByCount "15" @default.
• W2016097276 countsByYear W20160972762013 @default.
• W2016097276 countsByYear W20160972762015 @default.
• W2016097276 countsByYear W20160972762016 @default.
• W2016097276 countsByYear W20160972762018 @default.
• W2016097276 countsByYear W20160972762019 @default.
• W2016097276 countsByYear W20160972762020 @default.
• W2016097276 countsByYear W20160972762021 @default.
• W2016097276 countsByYear W20160972762022 @default.
• W2016097276 countsByYear W20160972762023 @default.
• W2016097276 crossrefType "journal-article" @default.
• W2016097276 hasAuthorship W2016097276A5008234020 @default.
• W2016097276 hasAuthorship W2016097276A5030685879 @default.
• W2016097276 hasAuthorship W2016097276A5032243491 @default.
• W2016097276 hasAuthorship W2016097276A5056148684 @default.
• W2016097276 hasAuthorship W2016097276A5073264020 @default.
• W2016097276 hasAuthorship W2016097276A5076520162 @default.
• W2016097276 hasAuthorship W2016097276A5081301991 @default.
• W2016097276 hasBestOaLocation W20160972762 @default.
• W2016097276 hasConcept C12554922 @default.
• W2016097276 hasConcept C133571119 @default.
• W2016097276 hasConcept C143937172 @default.
• W2016097276 hasConcept C150109051 @default.
• W2016097276 hasConcept C151730666 @default.
• W2016097276 hasConcept C181199279 @default.
• W2016097276 hasConcept C185592680 @default.
• W2016097276 hasConcept C2779343474 @default.
• W2016097276 hasConcept C2781307694 @default.
• W2016097276 hasConcept C45656491 @default.
• W2016097276 hasConcept C55493867 @default.
• W2016097276 hasConcept C71240020 @default.
• W2016097276 hasConcept C75599170 @default.
• W2016097276 hasConcept C8010536 @default.
• W2016097276 hasConcept C86803240 @default.
• W2016097276 hasConcept C97029542 @default.
• W2016097276 hasConceptScore W2016097276C12554922 @default.
• W2016097276 hasConceptScore W2016097276C133571119 @default.
• W2016097276 hasConceptScore W2016097276C143937172 @default.
• W2016097276 hasConceptScore W2016097276C150109051 @default.
• W2016097276 hasConceptScore W2016097276C151730666 @default.
• W2016097276 hasConceptScore W2016097276C181199279 @default.
• W2016097276 hasConceptScore W2016097276C185592680 @default.

• next