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- W2016098500 abstract "The dependence of the extent of DNA damage by anticancer bleomycin on pH and length of post-treatment incubation was studied in yeast. Bleomycin was always removed from cells after 20-min exposures, and cells were washed prior to incubation in non-nutrient buffer. Following exposures of late stationary-phase cells to the very low dose of only 3 μg/ml, 1.5 h incubation in non-nutrient buffer, pH 5, had hardly any effect on profiles derived from alkaline sucrose gradient sedimentation of nucleic acids released from spheroplasts. In contrast, after incubation of cells for 1.5 h in buffer, pH 7, DNA was all low molecular weight. Thus, even after extensive washing of cells, pH strongly influences the drug's action on DNA. At pH 5, washed cells were increasingly susceptible to DNA damage up to 26 h in non-nutrient buffer." @default.
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- W2016098500 date "1980-12-01" @default.
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- W2016098500 title "Influence of pH and length of post-treatment incubation on bleomycin-induced DNA damage" @default.
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- W2016098500 doi "https://doi.org/10.1016/0005-2787(80)90024-6" @default.
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