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- W2016112400 abstract "A fully automated ELISA system was constructed using a pipette tip as a solid phase, urease as a detecting enzyme, and a pH-FET as a detector of urease activity. The inner wall of the end part of a pipette tip was used as a solid phase, and the urease activity of the conjugate, captured after a two-step immunoreaction, was measured by coupling the pipette tip with the pH-FET in a pH-measuring cell. Full automation of the ELISA system was achieved by using a disposable reagent cartridge and three pipetters for all mechanical operations, including sample dilution and BF separation. This system can treat 60 samples per hour with an assay time of 21 min for all assay configurations. The system was applied to two-step sandwich assays for AFP, CEA, HBsAg, and HBsAb, a two-step competition assay for HBcAb, and a second antibody assay for HTLV-I Ab." @default.
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- W2016112400 date "1995-06-01" @default.
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- W2016112400 title "An automated ELISA system using a pipette tip as a solid phase and a pH-sensitive field effect transistor as a detector" @default.
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- W2016112400 doi "https://doi.org/10.1016/0022-1759(95)00058-i" @default.
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