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• W2016113156 abstract "We show that firefly luciferase is a stable protein when expressed at 25°C in Neurospora, which limits its use as transcription reporter. We created a short-lived luciferase by fusing a PEST signal to its C-terminus (LUC-PEST) and applied the LUC-PEST reporter system to record in vivo transcription dynamics associated with the Neurospora circadian clock and its blue-light photosensory system over the course of several days. We show that the tool is suitable to faithfully monitor rapid, but also subtle changes in transcription in a medium to high throughput format." @default.
• W2016113156 created "2016-06-24" @default.
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• W2016113156 date "2013-12-31" @default.
• W2016113156 modified "2023-09-26" @default.
• W2016113156 title "Light-Dependent and Circadian Transcription Dynamics In Vivo Recorded with a Destabilized Luciferase Reporter in Neurospora" @default.
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• W2016113156 doi "https://doi.org/10.1371/journal.pone.0083660" @default.
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