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- W2016126351 abstract "Solid-state 13C nuclear magnetic resonance (NMR) spectroscopy was applied to intact and isolated loblolly pine wood samples to identify potential structural changes induced by tree age, milling, lignin extraction, or naturally occurring mutations. Special attention was paid to ketone and aldehyde as well as nonpolar alkyl groups, which could be observed at low concentrations (<2 in 1000 C) using improved spinning-sideband suppression with gated decoupling. Carbonyl structures were present in intact wood, and there are more keto groups than aldehydes. Their concentrations increased from juvenile to mature wood and with milling time, whereas extraction did not alter the CO fraction. Significant amounts of aldehyde and dihydroconiferyl alcohol residues were present in coniferyl aldehyde dehydrogenase-deficient wood, confirming solution-state NMR spectra of the corresponding lignin. These results demonstrate the utility of solid-state NMR as an assay for changes in the lignin structure of genetically modified plants. Keywords: Lignin; lignin in wood; lignin isolation; milled wood lignin (MWL); solid-state NMR; spectral editing" @default.
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- W2016126351 title "Differences between Lignin in Unprocessed Wood, Milled Wood, Mutant Wood, and Extracted Lignin Detected by <sup>13</sup>C Solid-State NMR" @default.
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- W2016126351 doi "https://doi.org/10.1021/jf062199q" @default.
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