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- W201612738 abstract "miRNA prediction algorithms often fail to predict the accurate location of the mature miRNA in a precursor sequence with nucleotide-level precision. miRNAs-rapid amplification of cDNA ends (miR-RACE) is an efficient method to determine the precise sequences of computationally predicted microRNAs (miRNAs). miR-RACE includes the following steps: miRNA-enriched library preparation, two specific 5′- and 3′-miRNA RACE (miR-RACE) PCR reactions, and sequence-directed cloning. The most challenging step is the two gene-specific primers designed for the two RACE reactions. The miR-RACE protocol is rapid and can be executed and completed in 2–3 days." @default.
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- W201612738 date "2015-01-01" @default.
- W201612738 modified "2023-09-23" @default.
- W201612738 title "A Method for Validating MicroRNAs in Plants by miR-RACE" @default.
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- W201612738 doi "https://doi.org/10.1007/978-1-4939-2453-0_10" @default.
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