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• W2016144447 abstract "The suicide plasmid pfdA31-Tn5 was constructed to mutagenize Erwinia amylovora and Escherichia coli strains by electorporation. This vector carries the bacteriophage fd replication origin, a beta-lactamase gene and the transposon Tn5. For propagation the plasmid depends on host cells producing fd gene-2 protein. Electroporation of E.amylovora or E.coli cells with plasmid pfdA31-Tn5 yielded more than 10(4) transposition events per micrograms DNA. We have produced and characterized transposon mutants of E.amylovora affecting either galactose metabolism or the synthesis of the phytotoxin (L)-2,5-dihydrophenylalanine. A Tn5-insertion in a gene, involved in exopolysaccharide synthesis of E.amylovora strain Ea7/74, was subcloned into vector pfdA31 and used to mutagenize E.amylovora strain Ea1/79 by site-directed recombination." @default.
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• W2016144447 date "1992-01-01" @default.
• W2016144447 modified "2023-09-27" @default.
• W2016144447 title "Site-directed and transposon-mediated mutagenesis with pfd-plasmids by electroporation ofErwinia amylovoraandEscherichia colicells" @default.
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• W2016144447 doi "https://doi.org/10.1093/nar/20.9.2265" @default.
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