FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016159802> ?p ?o ?g. }

• W2016159802 abstract "Abstract Background Bacteriocin-producing lactic acid bacteria are commonly used as natural protective cultures. Among them, strains of the genus Pediococcus are particularly interesting for their ability to produce pediocin, a broad spectrum antimicrobial peptide with a strong antagonistic activity against the food-borne pathogen Listeria monocytogenes . Furthermore, there is increasing interest in isolating new bacteriocin-producing strains of human intestinal origin that could be developed for probiotic effects and inhibition of pathogenic bacteria in the gut. In this work, we typed a new strain, co-isolated from baby faeces together with a Bifidobacterium thermophilum strain, and characterized its proteinaceous compound with strong antilisterial activity. Results The newly isolated strain UVA1 was identified as a Pediococcus acidilactici by carbohydrate fermentation profile, growth at 50°C and 16S rDNA sequencing. The partially purified bacteriocin was heat resistant up to 100°C, active over a wide range of pH (2 to 9) and susceptible to proteolytic enzymes. The molecular weight, estimated by SDS-PAGE, was similar to that of pediocin AcH/PA-1 (4.5 kDa). P. acidilactici UVA1 harboured a 9.5-kb plasmid that could be cured easily, which resulted in the loss of the antimicrobial activity. Southern hybridization using the DIG-labelled pedA -probe established that the bacteriocin gene was plasmid-borne as for all pediocin described so far. Nucleotide sequence of the whole operon (3.5 kb) showed almost 100 % similarity to the pediocin AcH/PA-1 operon. The mRNA transcript for pedA could be detected in P. acidilactici UVA1 but not in the cured derivative, confirming the expression of the pedA -gene in UVA1. Using a new real-time PCR assay, eleven out of seventeen human faecal samples tested were found to contain pedA -DNA. Conclusion We identified and characterised the first pediocin produced by a human intestinal Pediococcus acidilactici isolate and successfully developed a new real-time PCR assay to show the large distribution of pedA -containing strains in baby faecal samples." @default.
• W2016159802 created "2016-06-24" @default.
• W2016159802 creator A5007856392 @default.
• W2016159802 creator A5031159410 @default.
• W2016159802 creator A5035766579 @default.
• W2016159802 creator A5045172839 @default.
• W2016159802 creator A5068540391 @default.
• W2016159802 creator A5071608663 @default.
• W2016159802 creator A5075505085 @default.
• W2016159802 date "2007-09-12" @default.
• W2016159802 modified "2023-10-16" @default.
• W2016159802 title "Detection of the pediocin gene pedA in strains from human faeces by real-time PCR and characterization of Pediococcus acidilacticiUVA1" @default.
• W2016159802 cites W1499500424 @default.
• W2016159802 cites W1510612702 @default.
• W2016159802 cites W1543344293 @default.
• W2016159802 cites W1964520619 @default.
• W2016159802 cites W1966111485 @default.
• W2016159802 cites W1967524951 @default.
• W2016159802 cites W1975085160 @default.
• W2016159802 cites W1986823589 @default.
• W2016159802 cites W1995119182 @default.
• W2016159802 cites W1999291944 @default.
• W2016159802 cites W2010154158 @default.
• W2016159802 cites W2032546849 @default.
• W2016159802 cites W2042016354 @default.
• W2016159802 cites W2045814624 @default.
• W2016159802 cites W2048768167 @default.
• W2016159802 cites W2050504883 @default.
• W2016159802 cites W2058328304 @default.
• W2016159802 cites W2061799513 @default.
• W2016159802 cites W2088807108 @default.
• W2016159802 cites W2092881083 @default.
• W2016159802 cites W2102274379 @default.
• W2016159802 cites W2104302689 @default.
• W2016159802 cites W2105018887 @default.
• W2016159802 cites W2105620360 @default.
• W2016159802 cites W2111891338 @default.
• W2016159802 cites W2119994555 @default.
• W2016159802 cites W2137295250 @default.
• W2016159802 cites W2139922719 @default.
• W2016159802 cites W2149050302 @default.
• W2016159802 cites W2161960172 @default.
• W2016159802 cites W2170917404 @default.
• W2016159802 cites W2170971911 @default.
• W2016159802 cites W2171159499 @default.
• W2016159802 cites W2783981376 @default.
• W2016159802 doi "https://doi.org/10.1186/1472-6750-7-55" @default.
• W2016159802 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2034554" @default.
• W2016159802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17850651" @default.
• W2016159802 hasPublicationYear "2007" @default.
• W2016159802 type Work @default.
• W2016159802 sameAs 2016159802 @default.
• W2016159802 citedByCount "27" @default.
• W2016159802 countsByYear W20161598022012 @default.
• W2016159802 countsByYear W20161598022013 @default.
• W2016159802 countsByYear W20161598022018 @default.
• W2016159802 countsByYear W20161598022019 @default.
• W2016159802 countsByYear W20161598022020 @default.
• W2016159802 countsByYear W20161598022021 @default.
• W2016159802 countsByYear W20161598022022 @default.
• W2016159802 crossrefType "journal-article" @default.
• W2016159802 hasAuthorship W2016159802A5007856392 @default.
• W2016159802 hasAuthorship W2016159802A5031159410 @default.
• W2016159802 hasAuthorship W2016159802A5035766579 @default.
• W2016159802 hasAuthorship W2016159802A5045172839 @default.
• W2016159802 hasAuthorship W2016159802A5068540391 @default.
• W2016159802 hasAuthorship W2016159802A5071608663 @default.
• W2016159802 hasAuthorship W2016159802A5075505085 @default.
• W2016159802 hasBestOaLocation W20161598021 @default.
• W2016159802 hasConcept C104317684 @default.
• W2016159802 hasConcept C115574571 @default.
• W2016159802 hasConcept C22744801 @default.
• W2016159802 hasConcept C2775920511 @default.
• W2016159802 hasConcept C2777388531 @default.
• W2016159802 hasConcept C2778985185 @default.
• W2016159802 hasConcept C2779044325 @default.
• W2016159802 hasConcept C2780206646 @default.
• W2016159802 hasConcept C4937899 @default.
• W2016159802 hasConcept C523546767 @default.
• W2016159802 hasConcept C54355233 @default.
• W2016159802 hasConcept C55493867 @default.
• W2016159802 hasConcept C86803240 @default.
• W2016159802 hasConcept C89423630 @default.
• W2016159802 hasConceptScore W2016159802C104317684 @default.
• W2016159802 hasConceptScore W2016159802C115574571 @default.
• W2016159802 hasConceptScore W2016159802C22744801 @default.
• W2016159802 hasConceptScore W2016159802C2775920511 @default.
• W2016159802 hasConceptScore W2016159802C2777388531 @default.
• W2016159802 hasConceptScore W2016159802C2778985185 @default.
• W2016159802 hasConceptScore W2016159802C2779044325 @default.
• W2016159802 hasConceptScore W2016159802C2780206646 @default.
• W2016159802 hasConceptScore W2016159802C4937899 @default.
• W2016159802 hasConceptScore W2016159802C523546767 @default.
• W2016159802 hasConceptScore W2016159802C54355233 @default.
• W2016159802 hasConceptScore W2016159802C55493867 @default.
• W2016159802 hasConceptScore W2016159802C86803240 @default.
• W2016159802 hasConceptScore W2016159802C89423630 @default.
• W2016159802 hasIssue "1" @default.
• W2016159802 hasLocation W20161598021 @default.
• W2016159802 hasLocation W20161598022 @default.

• next