FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016160070> ?p ?o ?g. }

• W2016160070 endingPage "979" @default.
• W2016160070 startingPage "971" @default.
• W2016160070 abstract "Abstract Microfluidic devices permit perfusion culture of three‐dimensional (3D) tissue, mimicking the flow of blood in vascularized 3D tissue in our body. Here, we report a microfluidic device composed of a two‐part microfluidic chamber chip and multi‐microwell array chip able to be disassembled at the culture endpoint. Within the microfluidic chamber, an array of 3D tissue aggregates (spheroids) can be formed and cultured under perfusion. Subsequently, detailed post‐culture analysis of the spheroids collected from the disassembled device can be performed. This device facilitates uniform spheroid formation, growth analysis in a high‐throughput format, controlled proliferation via perfusion flow rate, and post‐culture analysis of spheroids. We used the device to culture spheroids of human hepatocellular carcinoma (HepG2) cells under two controlled perfusion flow rates. HepG2 spheroids exhibited greater cell growth at higher perfusion flow rates than at lower perfusion flow rates, and exhibited different metabolic activity and mRNA and protein expression under the different flow rate conditions. These results show the potential of perfusion culture to precisely control the culture environment in microfluidic devices. The construction of spheroid array chambers allows multiple culture conditions to be tested simultaneously, with potential applications in toxicity and drug screening." @default.
• W2016160070 created "2016-06-24" @default.
• W2016160070 creator A5021215364 @default.
• W2016160070 creator A5044347263 @default.
• W2016160070 creator A5058060254 @default.
• W2016160070 creator A5063401247 @default.
• W2016160070 creator A5073988985 @default.
• W2016160070 creator A5086619153 @default.
• W2016160070 date "2014-06-12" @default.
• W2016160070 modified "2023-10-16" @default.
• W2016160070 title "Detachably assembled microfluidic device for perfusion culture and post-culture analysis of a spheroid array" @default.
• W2016160070 cites W1972023839 @default.
• W2016160070 cites W1979721854 @default.
• W2016160070 cites W1980460031 @default.
• W2016160070 cites W1988781086 @default.
• W2016160070 cites W1990903536 @default.
• W2016160070 cites W1993285435 @default.
• W2016160070 cites W2004875910 @default.
• W2016160070 cites W2008811229 @default.
• W2016160070 cites W2021351846 @default.
• W2016160070 cites W2022038342 @default.
• W2016160070 cites W2022880352 @default.
• W2016160070 cites W2026632078 @default.
• W2016160070 cites W2030635496 @default.
• W2016160070 cites W2033474014 @default.
• W2016160070 cites W2037738941 @default.
• W2016160070 cites W2045075844 @default.
• W2016160070 cites W2050942009 @default.
• W2016160070 cites W2054251094 @default.
• W2016160070 cites W2059707689 @default.
• W2016160070 cites W2062712545 @default.
• W2016160070 cites W2066482021 @default.
• W2016160070 cites W2069560291 @default.
• W2016160070 cites W2083799705 @default.
• W2016160070 cites W2102197759 @default.
• W2016160070 cites W2108349736 @default.
• W2016160070 cites W2117336213 @default.
• W2016160070 cites W2122671103 @default.
• W2016160070 cites W2126750664 @default.
• W2016160070 cites W2133031196 @default.
• W2016160070 cites W2140395408 @default.
• W2016160070 cites W2161263785 @default.
• W2016160070 doi "https://doi.org/10.1002/biot.201300559" @default.
• W2016160070 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24802801" @default.
• W2016160070 hasPublicationYear "2014" @default.
• W2016160070 type Work @default.
• W2016160070 sameAs 2016160070 @default.
• W2016160070 citedByCount "22" @default.
• W2016160070 countsByYear W20161600702014 @default.
• W2016160070 countsByYear W20161600702015 @default.
• W2016160070 countsByYear W20161600702016 @default.
• W2016160070 countsByYear W20161600702017 @default.
• W2016160070 countsByYear W20161600702018 @default.
• W2016160070 countsByYear W20161600702019 @default.
• W2016160070 countsByYear W20161600702020 @default.
• W2016160070 countsByYear W20161600702021 @default.
• W2016160070 countsByYear W20161600702022 @default.
• W2016160070 countsByYear W20161600702023 @default.
• W2016160070 crossrefType "journal-article" @default.
• W2016160070 hasAuthorship W2016160070A5021215364 @default.
• W2016160070 hasAuthorship W2016160070A5044347263 @default.
• W2016160070 hasAuthorship W2016160070A5058060254 @default.
• W2016160070 hasAuthorship W2016160070A5063401247 @default.
• W2016160070 hasAuthorship W2016160070A5073988985 @default.
• W2016160070 hasAuthorship W2016160070A5086619153 @default.
• W2016160070 hasConcept C121332964 @default.
• W2016160070 hasConcept C12554922 @default.
• W2016160070 hasConcept C126322002 @default.
• W2016160070 hasConcept C136229726 @default.
• W2016160070 hasConcept C146957229 @default.
• W2016160070 hasConcept C163688615 @default.
• W2016160070 hasConcept C171250308 @default.
• W2016160070 hasConcept C172120300 @default.
• W2016160070 hasConcept C175369904 @default.
• W2016160070 hasConcept C185592680 @default.
• W2016160070 hasConcept C192562407 @default.
• W2016160070 hasConcept C202751555 @default.
• W2016160070 hasConcept C54355233 @default.
• W2016160070 hasConcept C55493867 @default.
• W2016160070 hasConcept C57879066 @default.
• W2016160070 hasConcept C71924100 @default.
• W2016160070 hasConcept C81885089 @default.
• W2016160070 hasConcept C8673954 @default.
• W2016160070 hasConcept C86803240 @default.
• W2016160070 hasConcept C90703135 @default.
• W2016160070 hasConceptScore W2016160070C121332964 @default.
• W2016160070 hasConceptScore W2016160070C12554922 @default.
• W2016160070 hasConceptScore W2016160070C126322002 @default.
• W2016160070 hasConceptScore W2016160070C136229726 @default.
• W2016160070 hasConceptScore W2016160070C146957229 @default.
• W2016160070 hasConceptScore W2016160070C163688615 @default.
• W2016160070 hasConceptScore W2016160070C171250308 @default.
• W2016160070 hasConceptScore W2016160070C172120300 @default.
• W2016160070 hasConceptScore W2016160070C175369904 @default.
• W2016160070 hasConceptScore W2016160070C185592680 @default.
• W2016160070 hasConceptScore W2016160070C192562407 @default.
• W2016160070 hasConceptScore W2016160070C202751555 @default.
• W2016160070 hasConceptScore W2016160070C54355233 @default.

• next