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- W2016165328 abstract "Bioprostheses for cardiovascular surgery have limitations in their use following as calicification. α-galactosidase epitope is known as a stimulant of immune response and then shows a progressing calcification. The objective of this study was to evaluate histologic characteristics and mechanical properties of decellularization and treated with α-galactosidase.BOVINE PERICARDIAL TISSUES WERE ALLOCATED INTO THREE GROUPS: fixation only with glutaraldehyde, decellularization with sodium dodesyl sulfate and decellularization plus treatment with α-galactosidase. We confirmed immunohistological characteristics and mechanical properties as fatigue test, permeability test, compliance test, tensile strength (strain) test and thermal stability test.Decellularization and elimination of α-gal were confirmed through immunohistologic findings. Decellularization had decreased mechanical properties compared to fixation only group in permeability (before fatigue test p=0.02, after fatigue test p=0.034), compliance (after fatigue test p=0.041), and tensile strength test (p=0.00). The group of decellularization plus treatment with α-galactosidase had less desirable mechanical properties than the group of decellularization in concerns of permeability (before fatigue test p=0.043) and strain test (p=0.001).Favorable decellularization and elimination of α-gal were obtained in this study through immunohistologic findings. However, those treatment including decellularization and elimination of α-gal implied the decreased mechanical properties in specific ways. We need more study to complete appropriate ioprosthesis with decellularization and elimination of α-gal including favorable mechanical properties too." @default.
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- W2016165328 date "2012-12-07" @default.
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- W2016165328 title "Histologic Characteristics and Mechanical Properties of Bovine Pericardium Treated with Decellularization and α-Galactosidase: A Comparative Study" @default.
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- W2016165328 doi "https://doi.org/10.5090/kjtcs.2012.45.6.368" @default.
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