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- W2016171830 abstract "Infectious RNA from turnip yellow mosaic virus (TYMV) has been prepared by phenol extraction. The molecular weight determined by sedimentation and viscosity measurements is 2·3 ± 0·15 × 106, corresponding with the maximum molecular weight of RNA per particle. The heating of TYMV RNA to 60°C in 0·01 M-salt resulted in a rapid drop in molecular weight for a variable proportion of the RNA. The proportion of thermally stable RNA was found to be positively correlated with the infectivity of the virus from which the RNA was prepared. Virus inactivated by incubation in sap at 37°C prior to purification yielded RNA which contained no thermally stable molecules, and which was noninfectious. Zone centrifugation of heated, infectious RNA on a sucrose gradient indicated that nearly all the infectivity is present in the small fraction of thermally stable molecules, corresponding with M = 2·3 × 106. These data are consistent with two alternative hypotheses: infectious RNA consists of a single polynucleotide chain. Inactivation and thermal lability are caused by chain scission by degradative agents; infectious RNA consists of several polynucleotide chains crosslinked by a factor conferring thermal stability to the complex. Inactivation and thermal lability are caused by loss of this factor." @default.
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- W2016171830 date "1962-05-01" @default.
- W2016171830 modified "2023-09-23" @default.
- W2016171830 title "Studies on infectious RNA from turnip yellow mosaic virus" @default.
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- W2016171830 doi "https://doi.org/10.1016/s0022-2836(62)80016-3" @default.
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