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- W2016180548 abstract "Background Actin is essential for tip growth in plants. However, imaging actin in live plant cells has heretofore presented challenges. In previous studies, fluorescent probes derived from actin-binding proteins often alter growth, cause actin bundling and fail to resolve actin microfilaments. Methodology/Principal Findings In this report we use Lifeact-mEGFP, an actin probe that does not affect the dynamics of actin, to visualize actin in the moss Physcomitrella patens and pollen tubes from Lilium formosanum and Nicotiana tobaccum. Lifeact-mEGFP robustly labels actin microfilaments, particularly in the apex, in both moss protonemata and pollen tubes. Lifeact-mEGFP also labels filamentous actin structures in other moss cell types, including cells of the gametophore. Conclusions/Significance Lifeact-mEGFP, when expressed at optimal levels does not alter moss protonemal or pollen tube growth. We suggest that Lifeact-mEGFP represents an exciting new versatile probe for further studies of actin's role in tip growing plant cells." @default.
- W2016180548 created "2016-06-24" @default.
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- W2016180548 date "2009-05-29" @default.
- W2016180548 modified "2023-10-16" @default.
- W2016180548 title "Lifeact-mEGFP Reveals a Dynamic Apical F-Actin Network in Tip Growing Plant Cells" @default.
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- W2016180548 doi "https://doi.org/10.1371/journal.pone.0005744" @default.
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