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- W2016192714 abstract "(1) The intracellular pH (pHi) of superfused slices of guinea-pig cerebral cortex was measured in 31P-NMR spectra using the chemical shifts of intracellular inorganic phosphate (Pi) and of 2-deoxy-glucose 6-phosphate (DOG6P). The pHi was found to be 7.30±0.04 (SD, n = 15) in bicarbonate-buffered medium and 7.20±0.05 (n = 10, P < 0.001) in bicarbonate-free HEPES buffer of the same pH (7.4). (2) Decreases in pHe below 7.05 resulted in pHi falling to similar values, with a decrease in the energy state. There was no change in intracellular lactate as assessed by 1H-NMR. (3) The tissues showed an ability to buffer higher pH : increasing pHe to 8.0 had no effect on pHi, PCr or lactate. (4) In order to characterize possible mechanisms of pH regulation in the tissue, the recovery from acid insult was investigated under various conditions. Initially pHi was decreased to 6.44±0.15 (n = 15) by exposure to media containing 6 mM bicarbonate gassed with O2/CO2, 80:20 (pHe 6.4). When this medium was replaced by normal bicarbonate buffer (pH 7.4) there was full recovery of pHi to 7.31±0.05 (n = 15), whereas replacing the buffer with HEPES resulted in incomplete recovery of pHi to 6.88±0.15 (n = 15, P < 0.001). (5) In the presence of the carbonic anhydrase inhibitor, acetazolamide (1 mM), or the sodium/proton exchange inhibitor, amiloride (1 mM), there was an incomplete return of pHi to the control value (pHi 6.90±0.20, n = 5, P < 0.001). (6) There was partial recovery of pHi in the presence of an inhibitor of the sodium/bicarbonate co-transporter, 4,4′-diisothiocyanatostilbene-2,2′-disulphonic acid (DIDS), to 7.11±0.15 (n = 5, P < 0.02). (7) These results indicate that both exchangers, bicarbonate/chloride and sodium/proton, are involved in pHi regulation in the intact metabolizing cerebral cortex. The role of the sodium/bicarbonate co-transporter is less clear." @default.
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- W2016192714 date "1992-10-01" @default.
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- W2016192714 title "The regulation of intracellular pH studied by 31P- and 1H-NMR spectroscopy in superfused guinea-pig cerebral cortex slices" @default.
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- W2016192714 doi "https://doi.org/10.1016/0197-0186(92)90188-w" @default.
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