FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016194811> ?p ?o ?g. }

• W2016194811 endingPage "232" @default.
• W2016194811 startingPage "214" @default.
• W2016194811 abstract "Nonnucleate fragments of eggs of the sea urchinStrongylocentrotus purpuratus were made by sucrose density gradient centrifugation. The synthesis of RNA and of protein was studied in the nonnucleate fragments after artificial activation with butyric acid. Ethidium bromide inhibited the incorporation of uridine-5-3H into RNA by activated nonnucleate fragments and by cleaving embryos more effectively than did dactinomycin. The incorporation of uridine-5-3H into high molecular weight RNA was inhibited by 10 μg/ml of ethidium bromide by about 97% in nonnucleate fragments, 85% in fertilized eggs and 53% in blastulae. The electrophoretic pattern of proteins labeled in activated nonnucleate fragments, in sodium dodecyl sulfate polyacrylamide gels, was not qualitatively altered by 6 hours of preincubation with 10 μg/ml of ethidium bromide. The range of molecular weights for the labeled proteins was 9 to 40 thousand for those found in the 15,000g supernatant fraction of the homogenate and 6 to 60 thousand for those in the corresponding pellet. These results establish that most, or possibly all, of the types of proteins labeled after artificial activation of nonnucleate fragments are synthesized upon RNA templates which were present in the unfertilized egg. Continuous exposure to various concentrations of ethidium bromide affected development. Two micrograms per milliliter produced a prism stage larva with an abbreviated gut and 5 μg/ml arrested development at the blastula stage. At 10 μg/ml of development did not proceed beyond the 4- to 8-cell stage. When mesenchyme blastulae or gastrulae were treated with 10 μg/ml of ethidium bromide, development ceased, but the embryos continued to swim for at least 30 hours." @default.
• W2016194811 created "2016-06-24" @default.
• W2016194811 creator A5067297656 @default.
• W2016194811 creator A5073525159 @default.
• W2016194811 date "1971-02-01" @default.
• W2016194811 modified "2023-10-11" @default.
• W2016194811 title "Protein synthesis and development in the absence of cytoplasmic RNA synthesis in nonnucleate egg fragments and embryos of sea urchins: Effect of ethidium bromide" @default.
• W2016194811 cites W1956793061 @default.
• W2016194811 cites W1966532367 @default.
• W2016194811 cites W1968434470 @default.
• W2016194811 cites W1975107163 @default.
• W2016194811 cites W1985235286 @default.
• W2016194811 cites W1987654848 @default.
• W2016194811 cites W1988762801 @default.
• W2016194811 cites W1991784541 @default.
• W2016194811 cites W1995004462 @default.
• W2016194811 cites W1996456459 @default.
• W2016194811 cites W2003152735 @default.
• W2016194811 cites W2009397253 @default.
• W2016194811 cites W2010103763 @default.
• W2016194811 cites W2013762004 @default.
• W2016194811 cites W2018900997 @default.
• W2016194811 cites W2021504472 @default.
• W2016194811 cites W2025989516 @default.
• W2016194811 cites W2039152952 @default.
• W2016194811 cites W2054360302 @default.
• W2016194811 cites W2058157521 @default.
• W2016194811 cites W2059187032 @default.
• W2016194811 cites W2061033588 @default.
• W2016194811 cites W2061921824 @default.
• W2016194811 cites W2062763216 @default.
• W2016194811 cites W2065627978 @default.
• W2016194811 cites W2066353663 @default.
• W2016194811 cites W2067831996 @default.
• W2016194811 cites W2068360612 @default.
• W2016194811 cites W2069757749 @default.
• W2016194811 cites W2074889889 @default.
• W2016194811 cites W2080227993 @default.
• W2016194811 cites W2086659612 @default.
• W2016194811 cites W2089557229 @default.
• W2016194811 cites W2091901891 @default.
• W2016194811 cites W2242293214 @default.
• W2016194811 cites W2739818277 @default.
• W2016194811 doi "https://doi.org/10.1016/0012-1606(71)90096-0" @default.
• W2016194811 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5103257" @default.
• W2016194811 hasPublicationYear "1971" @default.
• W2016194811 type Work @default.
• W2016194811 sameAs 2016194811 @default.
• W2016194811 citedByCount "37" @default.
• W2016194811 countsByYear W20161948112014 @default.
• W2016194811 countsByYear W20161948112019 @default.
• W2016194811 crossrefType "journal-article" @default.
• W2016194811 hasAuthorship W2016194811A5067297656 @default.
• W2016194811 hasAuthorship W2016194811A5073525159 @default.
• W2016194811 hasConcept C104317684 @default.
• W2016194811 hasConcept C116084860 @default.
• W2016194811 hasConcept C129536746 @default.
• W2016194811 hasConcept C153911025 @default.
• W2016194811 hasConcept C2776907736 @default.
• W2016194811 hasConcept C2777569064 @default.
• W2016194811 hasConcept C2781259782 @default.
• W2016194811 hasConcept C3675279 @default.
• W2016194811 hasConcept C552990157 @default.
• W2016194811 hasConcept C55493867 @default.
• W2016194811 hasConcept C6047887 @default.
• W2016194811 hasConcept C67705224 @default.
• W2016194811 hasConcept C86803240 @default.
• W2016194811 hasConcept C87073359 @default.
• W2016194811 hasConceptScore W2016194811C104317684 @default.
• W2016194811 hasConceptScore W2016194811C116084860 @default.
• W2016194811 hasConceptScore W2016194811C129536746 @default.
• W2016194811 hasConceptScore W2016194811C153911025 @default.
• W2016194811 hasConceptScore W2016194811C2776907736 @default.
• W2016194811 hasConceptScore W2016194811C2777569064 @default.
• W2016194811 hasConceptScore W2016194811C2781259782 @default.
• W2016194811 hasConceptScore W2016194811C3675279 @default.
• W2016194811 hasConceptScore W2016194811C552990157 @default.
• W2016194811 hasConceptScore W2016194811C55493867 @default.
• W2016194811 hasConceptScore W2016194811C6047887 @default.
• W2016194811 hasConceptScore W2016194811C67705224 @default.
• W2016194811 hasConceptScore W2016194811C86803240 @default.
• W2016194811 hasConceptScore W2016194811C87073359 @default.
• W2016194811 hasIssue "2" @default.
• W2016194811 hasLocation W20161948111 @default.
• W2016194811 hasLocation W20161948112 @default.
• W2016194811 hasOpenAccess W2016194811 @default.
• W2016194811 hasPrimaryLocation W20161948111 @default.
• W2016194811 hasRelatedWork W1503335502 @default.
• W2016194811 hasRelatedWork W1984175761 @default.
• W2016194811 hasRelatedWork W1986853640 @default.
• W2016194811 hasRelatedWork W2021506754 @default.
• W2016194811 hasRelatedWork W2049314661 @default.
• W2016194811 hasRelatedWork W2055526599 @default.
• W2016194811 hasRelatedWork W2096539140 @default.
• W2016194811 hasRelatedWork W2100766089 @default.
• W2016194811 hasRelatedWork W2167733805 @default.
• W2016194811 hasRelatedWork W4254979709 @default.
• W2016194811 hasVolume "24" @default.

• next