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- W2016201822 abstract "Evaluation of the Berthelot colour reaction has shown that reagent sequence is an important determinant of the chromogenic response. Color is formed from various non-protein nitrogen substances first treated with alkaline hypochlorite followed by phenol reagent. The time lapse before addition of phenol reagent to alkaline hypochlorite-containing mixtures is critical. The high pH promotes the volatilization of ammonia from non-protein nitrogen solutions treated with alkaline hypochlorite. This loss can be prevented by adding phenol reagent before alkaline hypochlorite. The phenol-hypochlorite reagent sequence appears to enhance the specificity of Berthelot colour formation. This reaction provides a basis for measuring urea nitrogen concentration of serum or protein-free filtrates with a degree of reliability satisfactory for most routine or research purposes." @default.
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- W2016201822 date "1965-08-01" @default.
- W2016201822 modified "2023-09-27" @default.
- W2016201822 title "A study of the specificity of the Berthelot colour reaction" @default.
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- W2016201822 doi "https://doi.org/10.1016/0009-8981(65)90026-4" @default.
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