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• W2016204535 abstract "Four parameters related to ribosome production have been determined in Escherichia coli growing exponentially at rates between 0·1 and 1·4 generations per hour at 30 °C. (1) The fraction of the total transcript which is ribosomal RNA was measured by hybridization of pulse-labeled total cellular RNA to DNA from a transducing λ-phage carrying a complete set of rRNA genes (λ-dilv5). The relative rate of rRNA transcription increases from 0·27 to 0·67 over the range of growth rates studied. (2) The fraction of the total transcript from approximately 25% of the ribosomal protein genes was measured by hybridization of pulse-labeled RNA to DNA from a transducing phage which carries 14 of the ribosomal protein genes (λ-dspc1). The relative rate of r-protein messenger RNA does not vary with the growth rate. (3) The fraction of the total transcript which accumulates in mature ribosomes and transfer RNA was calculated from the rate of stable RNA accumulation and the total rate of RNA synthesis, measured by short-term kinetics of uptake of [3H]adenine, corrected for the specific activity of the ATP pool. The relative rate of stable RNA accumulation increases from 0·07 to 0·5 over the range of growth rates studied. (4) The amount of r-protein mRNA relative to the amount of total cellular RNA was determined by hybridization of increasing amounts of pulse-labeled RNA to limiting amounts of λ-dspc1 DNA. The amount of r-protein mRNA per ribosome is closely proportional to αr, the relative rate of r-protein synthesis. Correlations of the different parameters permit the following conclusions. The transcription of rRNA and r-protein mRNA does not vary in concert as a function of the growth rate. It indicates that different mechanisms are responsible for the control of these two types of ribosomal operons. The rate of transcription of rRNA is always greater than the rate of accumulation of rRNA. The difference is particularly pronounced at low growth rates; at μ = 0·1 generation per hour, only 30% of the newly made rRNA is incorporated into mature ribosomes and the rest is broken down. At high growth rates about 10% of the newly made rRNA is broken down. An approximate estimate of total mRNA transcription can be made from determinations of the relative rate of rRNA synthesis; total mRNA constitutes a decreasing fraction of the total transcript varying from 0·8 to 0·25 between μ = 0·1 and 1·4. After the calculation of the relative rate of total mRNA transcription, the fraction of the total mRNA which is r-protein mRNA can be drived. The result is that the rate of r-protein mRNA syntheis relative to total mRNA synthesis is nearly proportional to αr, also the amount of r-protein mRNA varies in proportion to αr, indicating that the rate of r-protein synthesis is regulated at the level of transcription in the same way as synthesis of bulk protein." @default.
• W2016204535 created "2016-06-24" @default.
• W2016204535 creator A5082562076 @default.
• W2016204535 date "1977-09-01" @default.
• W2016204535 modified "2023-10-13" @default.
• W2016204535 title "Regulation of ribosome production in Escherichia coli: Synthesis and stability of ribosomal RNA and of ribosomal protein messenger RNA at different growth rates" @default.
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• W2016204535 doi "https://doi.org/10.1016/0022-2836(77)90158-9" @default.
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