Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016205346> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2016205346 endingPage "530" @default.
- W2016205346 startingPage "517" @default.
- W2016205346 abstract "Chromatography of cardiac muscle and brain extracts on DEAE-cellulose resolved phosphotyrosyl-protein phosphatase activity into three fractions, termed Y-1, Y-2 and Y-3. These were eluted at 0.05, 0.15 and 0.3 m KCl, representing about 33, 55 and 12%, respectively, of the enzymatic activity recovered from the resin. Comparative studies demonstrated that the properties of phosphatases Y-1, Y-2 and Y-3 were distinctly different from those of previously identified phosphoseryl-protein phosphatases-1, -2, -3, and -4. Phosphatases Y-1, Y-2 and Y-3 were stimulated by EDTA and exhibited optimal activity at neutral pH. These properties were different from those of the two minor phosphotyrosyl-protein phosphatase activities associated with phosphoseryl-protein phosphatases-3, and -4, which were divalent cation dependent and exhibited optimal activity at alkaline pH. Further purification of phosphatase Y-2 from bovine heart has been carried out. The enzyme had a Mr = 65,000 (Stokes radius = 3.8 nm; s20,w0 = 4.1). Its activity was stimulated by 5- to 10-fold in the presence of EDTA (Ka = 15 μM) and was strongly inhibited by micromolar concentrations of vanadate. Phosphatase Y-2 was highly specific for phosphotyrosyl-IgG and -casein, and showed little activity toward phosphoseryl-casein, -phosphorylase a, phosphothreonyl-inhibitor-1 and p-nitrophenyl phosphate. The present studies indicate that phosphotyrosyl-protein phosphatase activity in animal tissues exists in multiple forms. The major active species are specific for phosphotyrosyl proteins and represent enzymes different from the known phosphoseryl-protein phosphatases and p-nitrophenyl phosphatases." @default.
- W2016205346 created "2016-06-24" @default.
- W2016205346 creator A5001671766 @default.
- W2016205346 creator A5004591046 @default.
- W2016205346 date "1983-10-01" @default.
- W2016205346 modified "2023-10-14" @default.
- W2016205346 title "Multiple forms of phosphotyrosyl- and phosphoseryl-protein phosphatase from cardiac muscle: Partial purification and characterization of an EDTA-stimulated phosphotyrosyl-protein phosphatase" @default.
- W2016205346 cites W148345225 @default.
- W2016205346 cites W1503367039 @default.
- W2016205346 cites W1505870408 @default.
- W2016205346 cites W1507734858 @default.
- W2016205346 cites W1520168265 @default.
- W2016205346 cites W1524333424 @default.
- W2016205346 cites W1534965462 @default.
- W2016205346 cites W1538276446 @default.
- W2016205346 cites W1544586151 @default.
- W2016205346 cites W1553326863 @default.
- W2016205346 cites W1566040275 @default.
- W2016205346 cites W1567148937 @default.
- W2016205346 cites W1578370366 @default.
- W2016205346 cites W1599070783 @default.
- W2016205346 cites W1607928467 @default.
- W2016205346 cites W1975611863 @default.
- W2016205346 cites W1976291370 @default.
- W2016205346 cites W1976508520 @default.
- W2016205346 cites W1989054182 @default.
- W2016205346 cites W1989714051 @default.
- W2016205346 cites W1997376701 @default.
- W2016205346 cites W2007722349 @default.
- W2016205346 cites W2013109496 @default.
- W2016205346 cites W2017360965 @default.
- W2016205346 cites W2018275786 @default.
- W2016205346 cites W2027722451 @default.
- W2016205346 cites W2034331456 @default.
- W2016205346 cites W2035727222 @default.
- W2016205346 cites W2037673705 @default.
- W2016205346 cites W2056770304 @default.
- W2016205346 cites W2057799558 @default.
- W2016205346 cites W2062483982 @default.
- W2016205346 cites W2073119121 @default.
- W2016205346 cites W2093463726 @default.
- W2016205346 cites W2106116485 @default.
- W2016205346 cites W2343603404 @default.
- W2016205346 cites W2799771226 @default.
- W2016205346 cites W31638798 @default.
- W2016205346 doi "https://doi.org/10.1016/0003-9861(83)90321-1" @default.
- W2016205346 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6314904" @default.
- W2016205346 hasPublicationYear "1983" @default.
- W2016205346 type Work @default.
- W2016205346 sameAs 2016205346 @default.
- W2016205346 citedByCount "43" @default.
- W2016205346 countsByYear W20162053462012 @default.
- W2016205346 countsByYear W20162053462014 @default.
- W2016205346 countsByYear W20162053462015 @default.
- W2016205346 countsByYear W20162053462021 @default.
- W2016205346 crossrefType "journal-article" @default.
- W2016205346 hasAuthorship W2016205346A5001671766 @default.
- W2016205346 hasAuthorship W2016205346A5004591046 @default.
- W2016205346 hasConcept C178666793 @default.
- W2016205346 hasConcept C181199279 @default.
- W2016205346 hasConcept C185592680 @default.
- W2016205346 hasConcept C21547065 @default.
- W2016205346 hasConcept C389152 @default.
- W2016205346 hasConcept C55493867 @default.
- W2016205346 hasConcept C85528070 @default.
- W2016205346 hasConcept C97920573 @default.
- W2016205346 hasConceptScore W2016205346C178666793 @default.
- W2016205346 hasConceptScore W2016205346C181199279 @default.
- W2016205346 hasConceptScore W2016205346C185592680 @default.
- W2016205346 hasConceptScore W2016205346C21547065 @default.
- W2016205346 hasConceptScore W2016205346C389152 @default.
- W2016205346 hasConceptScore W2016205346C55493867 @default.
- W2016205346 hasConceptScore W2016205346C85528070 @default.
- W2016205346 hasConceptScore W2016205346C97920573 @default.
- W2016205346 hasIssue "2" @default.
- W2016205346 hasLocation W20162053461 @default.
- W2016205346 hasLocation W20162053462 @default.
- W2016205346 hasOpenAccess W2016205346 @default.
- W2016205346 hasPrimaryLocation W20162053461 @default.
- W2016205346 hasRelatedWork W104091829 @default.
- W2016205346 hasRelatedWork W138614803 @default.
- W2016205346 hasRelatedWork W2011904545 @default.
- W2016205346 hasRelatedWork W2080899088 @default.
- W2016205346 hasRelatedWork W2109680237 @default.
- W2016205346 hasRelatedWork W2156376499 @default.
- W2016205346 hasRelatedWork W2159993024 @default.
- W2016205346 hasRelatedWork W2764205146 @default.
- W2016205346 hasRelatedWork W2463882859 @default.
- W2016205346 hasRelatedWork W2507843907 @default.
- W2016205346 hasVolume "226" @default.
- W2016205346 isParatext "false" @default.
- W2016205346 isRetracted "false" @default.
- W2016205346 magId "2016205346" @default.
- W2016205346 workType "article" @default.