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• W2016232482 abstract "Results of recent radioligand binding experiments suggest that chronic cocaine exposure increases dopamine transporter (DAT) synthesis throughout the striatum of humans. However, detection of cocaine binding site increases in animals and humans has varied depending on the radioligand used. The present experiment tested the hypothesis in cocaine-using humans that synthesis of midbrain DAT messenger RNA increases parallel with increased striatal DAT binding sites.Striatal and midbrain samples were collected during autopsy examination from human cocaine users (n = 34) and from age-, sex-, and race-matched control subjects (n = 36). Levels of DAT messenger RNA were quantified in the medial and lateral midbrain regions using in situ hybridization, and striatal DAT binding sites were assessed by quantitative autoradiography using the DAT-specific radioligand [3H]WIN 35428.Striatal DAT binding sites were markedly increased in cocaine users, but, paradoxically, medial DAT messenger RNA levels were decreased.Cocaine exposure has a marked effect on DAT function, but the mechanisms involved may be complex." @default.
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• W2016232482 date "1998-09-01" @default.
• W2016232482 modified "2023-10-13" @default.
• W2016232482 title "Brain Dopamine Transporter Messenger RNA and Binding Sites in Cocaine Users" @default.
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• W2016232482 doi "https://doi.org/10.1001/archpsyc.55.9.793" @default.
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