Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016241331> ?p ?o ?g. }
- W2016241331 endingPage "237" @default.
- W2016241331 startingPage "227" @default.
- W2016241331 abstract "A qualitative and quantitative analysis of the conformation of Langmuir-Blodgett (LB) dried films of cytochrome C on silicon wafers was performed by Fourier transform ir (FTIR) spectroscopy. A deconvolution procedure was applied to the amide I band analysis, in order to determine the percentage of the different secondary structures. Qualitative analysis was performed by examining difference spectra. Films obtained by spreading protein solutions at pH 7.4 and 1, dried at 25 and 100 degrees C, on silicon wafers were also examined in order to detect spectral components associated with denatured protein domains, and to compare them with cytochrome C LB films. FTIR spectroscopy showed that the following important changes characterise LB film spectra: (a) the alpha-helix component is higher (its percentage is 57 and 54%) than the one estimated in dried film obtained by spreading the solutions at pH 7.4 on a silicon substrate (43%), (b) there is an increase in the intensity of bands attributed to protonated carboxy group bands, involved and not involved in the formation of hydrogen bonds, and a decrease in those attributed to deprotonated carboxy groups, (c) the intensity of several bands attributed to aromatic amino acids and aliphatic chains increases, and (d) bands due to O-H stretching vibrations of crystallization water are present. These conformational changes could be induced by protein-protein interaction caused by the close packing of molecules that occurs during LB film formation; it cannot be excluded that they may be accompanied by partial changes in the tertiary structure of the protein. A preferential orientation of protein molecules in LB films is also a possibility." @default.
- W2016241331 created "2016-06-24" @default.
- W2016241331 creator A5005088150 @default.
- W2016241331 creator A5005337706 @default.
- W2016241331 creator A5017424226 @default.
- W2016241331 creator A5047179112 @default.
- W2016241331 creator A5050037195 @default.
- W2016241331 creator A5066961829 @default.
- W2016241331 creator A5087906790 @default.
- W2016241331 date "1997-08-01" @default.
- W2016241331 modified "2023-10-16" @default.
- W2016241331 title "Qualitative and quantitative analysis of the secondary structure of cytochrome C Langmuir-Blodgett films" @default.
- W2016241331 cites W1934853983 @default.
- W2016241331 cites W1969898499 @default.
- W2016241331 cites W1977310106 @default.
- W2016241331 cites W1977843374 @default.
- W2016241331 cites W1987205935 @default.
- W2016241331 cites W1988968017 @default.
- W2016241331 cites W2000021330 @default.
- W2016241331 cites W2003027077 @default.
- W2016241331 cites W2010920019 @default.
- W2016241331 cites W2012453384 @default.
- W2016241331 cites W2014093665 @default.
- W2016241331 cites W2016106974 @default.
- W2016241331 cites W2021128357 @default.
- W2016241331 cites W2022862113 @default.
- W2016241331 cites W2024709897 @default.
- W2016241331 cites W2031737124 @default.
- W2016241331 cites W2035359684 @default.
- W2016241331 cites W2038396939 @default.
- W2016241331 cites W2045356176 @default.
- W2016241331 cites W2047039535 @default.
- W2016241331 cites W2048475498 @default.
- W2016241331 cites W2059634416 @default.
- W2016241331 cites W2062102053 @default.
- W2016241331 cites W2082389960 @default.
- W2016241331 cites W2083563479 @default.
- W2016241331 cites W2085365687 @default.
- W2016241331 cites W2086580653 @default.
- W2016241331 cites W2088107416 @default.
- W2016241331 cites W2092059009 @default.
- W2016241331 cites W2092372554 @default.
- W2016241331 cites W2094077114 @default.
- W2016241331 cites W2159426997 @default.
- W2016241331 cites W2323558655 @default.
- W2016241331 cites W2325795914 @default.
- W2016241331 cites W3104573845 @default.
- W2016241331 cites W345458310 @default.
- W2016241331 cites W76041064 @default.
- W2016241331 doi "https://doi.org/10.1002/(sici)1097-0282(199708)42:2<227::aid-bip11>3.0.co;2-i" @default.
- W2016241331 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9235001" @default.
- W2016241331 hasPublicationYear "1997" @default.
- W2016241331 type Work @default.
- W2016241331 sameAs 2016241331 @default.
- W2016241331 citedByCount "15" @default.
- W2016241331 countsByYear W20162413312014 @default.
- W2016241331 countsByYear W20162413312015 @default.
- W2016241331 countsByYear W20162413312017 @default.
- W2016241331 countsByYear W20162413312020 @default.
- W2016241331 crossrefType "journal-article" @default.
- W2016241331 hasAuthorship W2016241331A5005088150 @default.
- W2016241331 hasAuthorship W2016241331A5005337706 @default.
- W2016241331 hasAuthorship W2016241331A5017424226 @default.
- W2016241331 hasAuthorship W2016241331A5047179112 @default.
- W2016241331 hasAuthorship W2016241331A5050037195 @default.
- W2016241331 hasAuthorship W2016241331A5066961829 @default.
- W2016241331 hasAuthorship W2016241331A5087906790 @default.
- W2016241331 hasConcept C111368507 @default.
- W2016241331 hasConcept C112887158 @default.
- W2016241331 hasConcept C113196181 @default.
- W2016241331 hasConcept C127313418 @default.
- W2016241331 hasConcept C127413603 @default.
- W2016241331 hasConcept C145148216 @default.
- W2016241331 hasConcept C153642686 @default.
- W2016241331 hasConcept C160892712 @default.
- W2016241331 hasConcept C178790620 @default.
- W2016241331 hasConcept C185592680 @default.
- W2016241331 hasConcept C204921945 @default.
- W2016241331 hasConcept C2777289219 @default.
- W2016241331 hasConcept C28859421 @default.
- W2016241331 hasConcept C29311851 @default.
- W2016241331 hasConcept C30095370 @default.
- W2016241331 hasConcept C32909587 @default.
- W2016241331 hasConcept C42360764 @default.
- W2016241331 hasConcept C521977710 @default.
- W2016241331 hasConcept C544956773 @default.
- W2016241331 hasConcept C55493867 @default.
- W2016241331 hasConcept C62614982 @default.
- W2016241331 hasConcept C8010536 @default.
- W2016241331 hasConceptScore W2016241331C111368507 @default.
- W2016241331 hasConceptScore W2016241331C112887158 @default.
- W2016241331 hasConceptScore W2016241331C113196181 @default.
- W2016241331 hasConceptScore W2016241331C127313418 @default.
- W2016241331 hasConceptScore W2016241331C127413603 @default.
- W2016241331 hasConceptScore W2016241331C145148216 @default.
- W2016241331 hasConceptScore W2016241331C153642686 @default.
- W2016241331 hasConceptScore W2016241331C160892712 @default.
- W2016241331 hasConceptScore W2016241331C178790620 @default.