Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016250017> ?p ?o ?g. }
- W2016250017 endingPage "46" @default.
- W2016250017 startingPage "35" @default.
- W2016250017 abstract "RNA interference appears to have a great potential not only as an in vitro target validation, but also as a novel therapeutic strategy based on the highly specific and efficient silencing of a target gene. We hypothesize that MMP-9 siRNA can be effective as an MMP-9 protein inhibitor in a rat focal ischemia model. Male Sprague–Dawley rats (156) were subjected to 2 h of middle cerebral artery occlusion (by using the suture insertion method) followed by 24 h of reperfusion. In the treatment group, 5 μl MMP-9 siRNA was administrated by intracerebroventricular injection within 60 min after 2 h of focal ischemia. The siRNA transfection was demonstrated by fluorescence conjugated siRNA. Treatment with MMP-9 siRNA produced a significant reduction in the cerebral infarction volume, brain water content, mortality rate and accompanying neurological deficits. The followings were recorded: Evan's blue and IgG extravasation were reduced; the expression of MMP-9 mRNA and protein were significantly silenced; and immunohistochemistry and Western blot analysis revealed that the expression of MMP-9 and VEGF were reduced while occludin and collagen-IV were up-regulated in brain tissues. Our findings provide evidence that a liposomal formulation of siRNA might be used in vivo to silence the MMP-9 gene and could potentially serve as an important therapeutic alternative in patients with cerebral ischemia." @default.
- W2016250017 created "2016-06-24" @default.
- W2016250017 creator A5000644382 @default.
- W2016250017 creator A5011634639 @default.
- W2016250017 creator A5012307033 @default.
- W2016250017 creator A5016467704 @default.
- W2016250017 creator A5022411133 @default.
- W2016250017 creator A5028263710 @default.
- W2016250017 creator A5029525859 @default.
- W2016250017 creator A5033098099 @default.
- W2016250017 creator A5034704065 @default.
- W2016250017 creator A5056891933 @default.
- W2016250017 creator A5064315255 @default.
- W2016250017 creator A5077606232 @default.
- W2016250017 creator A5078143614 @default.
- W2016250017 creator A5083097750 @default.
- W2016250017 date "2009-03-01" @default.
- W2016250017 modified "2023-10-16" @default.
- W2016250017 title "Therapeutic application of gene silencing MMP-9 in a middle cerebral artery occlusion-induced focal ischemia rat model" @default.
- W2016250017 cites W1574172480 @default.
- W2016250017 cites W1846608835 @default.
- W2016250017 cites W1894541755 @default.
- W2016250017 cites W1955736456 @default.
- W2016250017 cites W1975448513 @default.
- W2016250017 cites W1976807374 @default.
- W2016250017 cites W1984047231 @default.
- W2016250017 cites W1984759940 @default.
- W2016250017 cites W1990711278 @default.
- W2016250017 cites W1996163490 @default.
- W2016250017 cites W2000292756 @default.
- W2016250017 cites W2001836007 @default.
- W2016250017 cites W2005675003 @default.
- W2016250017 cites W2012547585 @default.
- W2016250017 cites W2015615473 @default.
- W2016250017 cites W2017597607 @default.
- W2016250017 cites W2020923434 @default.
- W2016250017 cites W2021611419 @default.
- W2016250017 cites W2021905258 @default.
- W2016250017 cites W2023250693 @default.
- W2016250017 cites W2026950582 @default.
- W2016250017 cites W2030613585 @default.
- W2016250017 cites W2031897866 @default.
- W2016250017 cites W2033966887 @default.
- W2016250017 cites W2036489895 @default.
- W2016250017 cites W2038707465 @default.
- W2016250017 cites W2039598753 @default.
- W2016250017 cites W2039602397 @default.
- W2016250017 cites W2041455809 @default.
- W2016250017 cites W2046329404 @default.
- W2016250017 cites W2048047884 @default.
- W2016250017 cites W2052269200 @default.
- W2016250017 cites W2052312685 @default.
- W2016250017 cites W2060430044 @default.
- W2016250017 cites W2068984659 @default.
- W2016250017 cites W2073923875 @default.
- W2016250017 cites W2074895826 @default.
- W2016250017 cites W2087523642 @default.
- W2016250017 cites W2094024073 @default.
- W2016250017 cites W2096318291 @default.
- W2016250017 cites W2097131232 @default.
- W2016250017 cites W2101957440 @default.
- W2016250017 cites W2103975017 @default.
- W2016250017 cites W2105028929 @default.
- W2016250017 cites W2106361045 @default.
- W2016250017 cites W2108330459 @default.
- W2016250017 cites W2111066814 @default.
- W2016250017 cites W2111476808 @default.
- W2016250017 cites W2113892795 @default.
- W2016250017 cites W2126630077 @default.
- W2016250017 cites W2143167573 @default.
- W2016250017 cites W2143453317 @default.
- W2016250017 cites W2148992136 @default.
- W2016250017 cites W2158144219 @default.
- W2016250017 cites W2167892546 @default.
- W2016250017 cites W2501836428 @default.
- W2016250017 cites W2803499602 @default.
- W2016250017 doi "https://doi.org/10.1016/j.expneurol.2008.11.007" @default.
- W2016250017 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19073180" @default.
- W2016250017 hasPublicationYear "2009" @default.
- W2016250017 type Work @default.
- W2016250017 sameAs 2016250017 @default.
- W2016250017 citedByCount "83" @default.
- W2016250017 countsByYear W20162500172012 @default.
- W2016250017 countsByYear W20162500172013 @default.
- W2016250017 countsByYear W20162500172014 @default.
- W2016250017 countsByYear W20162500172015 @default.
- W2016250017 countsByYear W20162500172016 @default.
- W2016250017 countsByYear W20162500172017 @default.
- W2016250017 countsByYear W20162500172018 @default.
- W2016250017 countsByYear W20162500172019 @default.
- W2016250017 countsByYear W20162500172020 @default.
- W2016250017 countsByYear W20162500172021 @default.
- W2016250017 crossrefType "journal-article" @default.
- W2016250017 hasAuthorship W2016250017A5000644382 @default.
- W2016250017 hasAuthorship W2016250017A5011634639 @default.
- W2016250017 hasAuthorship W2016250017A5012307033 @default.
- W2016250017 hasAuthorship W2016250017A5016467704 @default.
- W2016250017 hasAuthorship W2016250017A5022411133 @default.