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- W2016261567 abstract "Abstract A simple and rapid procedure is proposed for the preparation of Fab from bovine IgG which is used as a single absorbent (removal of anti-light chain antibodies) in the production of class specific antisera anti γ (IgG), anti μ (IgM) and anti α (IgA). The papain digest is chromatographed on DEAE-cellulose equilibrated with 0·01 M PO 4 pH 8·0, then rechromatographed on DEAE-cellulose with 0·01 M Tris-HCl pH 8·6 + 0·05 M NaCl, then precipitated with ammonium sulfate at 60% saturation. The supernatant contains Fab which is free of the Fc piece and of intact IgG." @default.
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- W2016261567 date "1975-03-01" @default.
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- W2016261567 title "A simple procedure for the production of Fab from bovine IgG as an absorbent in the preparation of class-specific anti-immunoglobulin" @default.
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- W2016261567 doi "https://doi.org/10.1016/0019-2791(75)90237-2" @default.
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