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- W2016264692 abstract "Simplified procedures for immunoelectron microscopy (IEM) and electron microscopy (EM) are described. The procedures employ the principle of agar filtration and pseudoreplication. The modification consisted of the use of microwells for storage of gels with or without antiserum (for IEM or EM, respectively) and an array of containers in which pseudoreplication and negative staining were performed. The containers were prepared from 5 ml syringes from which the needle holding parts were cut. This device enabled simultaneous and rapid handling of specimens. With Sindbis virus as a model, our microwell pseudoreplica IEM (MW-PR-IEM) was compared to six other IEM techniques and was found to be the most rapid and sensitive technique. With the MW-PR-IEM technique, the specific minimal detection limit (detection of clumps) was 1.5 × 107 virus particles per ml, and the non-specific detection limit (detection of single virions) was 1.8 × 106virus particles per ml." @default.
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- W2016264692 date "1984-11-01" @default.
- W2016264692 modified "2023-09-26" @default.
- W2016264692 title "A simplified microwell pseudoreplica for the detection of viruses by electron microscopy and immunoelectron microscopy" @default.
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- W2016264692 doi "https://doi.org/10.1016/0166-0934(84)90024-7" @default.
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