FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016266986> ?p ?o ?g. }

• W2016266986 endingPage "239" @default.
• W2016266986 startingPage "229" @default.
• W2016266986 abstract "In the absence of tissue culture, electron microscopy or assays for viral antigen, the direct detection of hepatitis C virus (HCV) is by necessity dependent upon nucleic acid hybridisation methods. Of the available methods, amplification of HCV cDNA by polymerase chain reaction (PCR) commends itself by virtue of its extreme sensitivity and its consequent ability to detect the very low levels of HCV-RNA that are present in many clinical samples. In this review the development and evolution of PCR techniques for HCV detection are described and a number of clinical applications are considered in detail. The applications include diagnosis of acute infection during the seronegative window period prior to the appearance of HCV antibodies, and diagnosis of HCV infection in the immunosuppressed. PCR also enables identification of the chronic viraemic carrier state and it permits accurate monitoring of the antiviral effects of drugs such as interferon. Confirmation of the specificity of HCV antibody assays and detection of HCV contamination of blood donations and blood products are other important areas in which PCR techniques have proved invaluable. In addition, PCR-based techniques underlie an increasing number of molecular epidemiological and genotyping studies and they are providing insights into the details of HCV cellular tropism and replication. A number of logistic problems and operational difficulties are also discussed. Despite these limitations it is concluded that PCR will continue to make significant contributions to both clinical practice and to our understanding of the basic biology of HCV infection." @default.
• W2016266986 created "2016-06-24" @default.
• W2016266986 creator A5025552110 @default.
• W2016266986 date "1994-07-01" @default.
• W2016266986 modified "2023-10-14" @default.
• W2016266986 title "The polymerase chain reaction and hepatitis C virus diagnosis" @default.
• W2016266986 cites W1654040128 @default.
• W2016266986 cites W1967333777 @default.
• W2016266986 cites W1969130283 @default.
• W2016266986 cites W1974879228 @default.
• W2016266986 cites W1977733324 @default.
• W2016266986 cites W1980409482 @default.
• W2016266986 cites W1987834144 @default.
• W2016266986 cites W1989238097 @default.
• W2016266986 cites W1990282182 @default.
• W2016266986 cites W1991501340 @default.
• W2016266986 cites W1991524663 @default.
• W2016266986 cites W1992474194 @default.
• W2016266986 cites W1993023565 @default.
• W2016266986 cites W1996731902 @default.
• W2016266986 cites W1997282583 @default.
• W2016266986 cites W2000124886 @default.
• W2016266986 cites W2004339905 @default.
• W2016266986 cites W2005044200 @default.
• W2016266986 cites W2017835467 @default.
• W2016266986 cites W2019943793 @default.
• W2016266986 cites W2024462802 @default.
• W2016266986 cites W2027655764 @default.
• W2016266986 cites W2028877573 @default.
• W2016266986 cites W2030041350 @default.
• W2016266986 cites W2031465792 @default.
• W2016266986 cites W2033199408 @default.
• W2016266986 cites W2041577235 @default.
• W2016266986 cites W2042038647 @default.
• W2016266986 cites W2043475480 @default.
• W2016266986 cites W2046556299 @default.
• W2016266986 cites W2047480444 @default.
• W2016266986 cites W2047963114 @default.
• W2016266986 cites W2050717506 @default.
• W2016266986 cites W2052945913 @default.
• W2016266986 cites W2054796247 @default.
• W2016266986 cites W2055031628 @default.
• W2016266986 cites W2066580123 @default.
• W2016266986 cites W2067490538 @default.
• W2016266986 cites W2071759803 @default.
• W2016266986 cites W2079020481 @default.
• W2016266986 cites W2083765130 @default.
• W2016266986 cites W2084583343 @default.
• W2016266986 cites W2087431613 @default.
• W2016266986 cites W2090162548 @default.
• W2016266986 cites W2091835868 @default.
• W2016266986 cites W2092306879 @default.
• W2016266986 cites W2092733829 @default.
• W2016266986 cites W2093155194 @default.
• W2016266986 cites W2097063931 @default.
• W2016266986 cites W2097458469 @default.
• W2016266986 cites W2101762590 @default.
• W2016266986 cites W2107414185 @default.
• W2016266986 cites W2108696732 @default.
• W2016266986 cites W2118790215 @default.
• W2016266986 cites W2120287285 @default.
• W2016266986 cites W2121590197 @default.
• W2016266986 cites W2131187976 @default.
• W2016266986 cites W2132537748 @default.
• W2016266986 cites W2133487025 @default.
• W2016266986 cites W2134364227 @default.
• W2016266986 cites W2148657163 @default.
• W2016266986 cites W2149127345 @default.
• W2016266986 cites W2154168388 @default.
• W2016266986 cites W2154600216 @default.
• W2016266986 cites W2155247751 @default.
• W2016266986 cites W2155765347 @default.
• W2016266986 cites W2163131045 @default.
• W2016266986 cites W2164049708 @default.
• W2016266986 cites W2166229810 @default.
• W2016266986 cites W22122639 @default.
• W2016266986 cites W2324507396 @default.
• W2016266986 cites W2339016944 @default.
• W2016266986 cites W4229955267 @default.
• W2016266986 cites W4294216491 @default.
• W2016266986 doi "https://doi.org/10.1111/j.1574-6976.1994.tb00093.x" @default.
• W2016266986 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7522021" @default.
• W2016266986 hasPublicationYear "1994" @default.
• W2016266986 type Work @default.
• W2016266986 sameAs 2016266986 @default.
• W2016266986 citedByCount "21" @default.
• W2016266986 crossrefType "journal-article" @default.
• W2016266986 hasAuthorship W2016266986A5025552110 @default.
• W2016266986 hasBestOaLocation W20162669861 @default.
• W2016266986 hasConcept C104317684 @default.
• W2016266986 hasConcept C135763542 @default.
• W2016266986 hasConcept C159047783 @default.
• W2016266986 hasConcept C179786068 @default.
• W2016266986 hasConcept C203014093 @default.
• W2016266986 hasConcept C2522874641 @default.
• W2016266986 hasConcept C2776408679 @default.
• W2016266986 hasConcept C2776455275 @default.
• W2016266986 hasConcept C2777510210 @default.

• next