FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016274034> ?p ?o ?g. }

• W2016274034 endingPage "915" @default.
• W2016274034 startingPage "906" @default.
• W2016274034 abstract "New results are presented on the equilibria and kinetics of the ion exchange onto CM-Sephadex of polygalacturonase (pectinase) produced by the fermentation of Kluyveromyces marxianus. It is found that the equilibrium behavior follows the form of the Langmuir isotherm; the equilibrium is strongly affected by pH. High partitioning onto the ion-exchange matrix, with good retention of enzyme activity, is achieved in the pH range 3.5-5.0, and this can be qualitatively explained in terms of simple models for protein adsorption by ion exchange. The kinetics of ion exchange is modeled by assuming that the transfer resistances can be lumped into a single coefficient, and the results show that this gives a reasonable description of the adsorption kinetics. Under optimum conditions protein adsorption is enhanced by electrostatic effects and is extremely fast, and it is suggested that in these circumstances external mass transfer resistance is significant. At pH values close to the isoelectric point, electrostatic interactions are weak and intraparticle diffusion is rate-limiting: pore-blocking by adsorbed proteins appears to be important under these conditions. The results also provide the basis for an efficient single-step purification scheme." @default.
• W2016274034 created "2016-06-24" @default.
• W2016274034 creator A5013390920 @default.
• W2016274034 creator A5052811507 @default.
• W2016274034 creator A5064686826 @default.
• W2016274034 date "1993-11-01" @default.
• W2016274034 modified "2023-09-26" @default.
• W2016274034 title "Adsorption of Kluyveromyces marxianus pectinase on CM-Sephadex gels" @default.
• W2016274034 cites W1775749144 @default.
• W2016274034 cites W1973577131 @default.
• W2016274034 cites W1988224781 @default.
• W2016274034 cites W2009137806 @default.
• W2016274034 cites W2013150462 @default.
• W2016274034 cites W2018625441 @default.
• W2016274034 cites W2019836391 @default.
• W2016274034 cites W2027592518 @default.
• W2016274034 cites W2030163787 @default.
• W2016274034 cites W2034974405 @default.
• W2016274034 cites W2040999039 @default.
• W2016274034 cites W2061147835 @default.
• W2016274034 cites W2086431382 @default.
• W2016274034 cites W2091827458 @default.
• W2016274034 cites W2102131116 @default.
• W2016274034 cites W2111444253 @default.
• W2016274034 cites W2146536222 @default.
• W2016274034 cites W2155551443 @default.
• W2016274034 cites W2314104404 @default.
• W2016274034 doi "https://doi.org/10.1016/0141-0229(93)90165-x" @default.
• W2016274034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7694598" @default.
• W2016274034 hasPublicationYear "1993" @default.
• W2016274034 type Work @default.
• W2016274034 sameAs 2016274034 @default.
• W2016274034 citedByCount "7" @default.
• W2016274034 countsByYear W20162740342019 @default.
• W2016274034 crossrefType "journal-article" @default.
• W2016274034 hasAuthorship W2016274034A5013390920 @default.
• W2016274034 hasAuthorship W2016274034A5052811507 @default.
• W2016274034 hasAuthorship W2016274034A5064686826 @default.
• W2016274034 hasConcept C117760992 @default.
• W2016274034 hasConcept C121332964 @default.
• W2016274034 hasConcept C127413603 @default.
• W2016274034 hasConcept C145148216 @default.
• W2016274034 hasConcept C147789679 @default.
• W2016274034 hasConcept C148898269 @default.
• W2016274034 hasConcept C150394285 @default.
• W2016274034 hasConcept C172854840 @default.
• W2016274034 hasConcept C178790620 @default.
• W2016274034 hasConcept C181199279 @default.
• W2016274034 hasConcept C185592680 @default.
• W2016274034 hasConcept C2776917750 @default.
• W2016274034 hasConcept C2777576037 @default.
• W2016274034 hasConcept C2779222958 @default.
• W2016274034 hasConcept C2780764520 @default.
• W2016274034 hasConcept C42360764 @default.
• W2016274034 hasConcept C43617362 @default.
• W2016274034 hasConcept C51038369 @default.
• W2016274034 hasConcept C55493867 @default.
• W2016274034 hasConcept C62520636 @default.
• W2016274034 hasConcept C69357855 @default.
• W2016274034 hasConcept C96294017 @default.
• W2016274034 hasConcept C97355855 @default.
• W2016274034 hasConceptScore W2016274034C117760992 @default.
• W2016274034 hasConceptScore W2016274034C121332964 @default.
• W2016274034 hasConceptScore W2016274034C127413603 @default.
• W2016274034 hasConceptScore W2016274034C145148216 @default.
• W2016274034 hasConceptScore W2016274034C147789679 @default.
• W2016274034 hasConceptScore W2016274034C148898269 @default.
• W2016274034 hasConceptScore W2016274034C150394285 @default.
• W2016274034 hasConceptScore W2016274034C172854840 @default.
• W2016274034 hasConceptScore W2016274034C178790620 @default.
• W2016274034 hasConceptScore W2016274034C181199279 @default.
• W2016274034 hasConceptScore W2016274034C185592680 @default.
• W2016274034 hasConceptScore W2016274034C2776917750 @default.
• W2016274034 hasConceptScore W2016274034C2777576037 @default.
• W2016274034 hasConceptScore W2016274034C2779222958 @default.
• W2016274034 hasConceptScore W2016274034C2780764520 @default.
• W2016274034 hasConceptScore W2016274034C42360764 @default.
• W2016274034 hasConceptScore W2016274034C43617362 @default.
• W2016274034 hasConceptScore W2016274034C51038369 @default.
• W2016274034 hasConceptScore W2016274034C55493867 @default.
• W2016274034 hasConceptScore W2016274034C62520636 @default.
• W2016274034 hasConceptScore W2016274034C69357855 @default.
• W2016274034 hasConceptScore W2016274034C96294017 @default.
• W2016274034 hasConceptScore W2016274034C97355855 @default.
• W2016274034 hasIssue "11" @default.
• W2016274034 hasLocation W20162740341 @default.
• W2016274034 hasLocation W20162740342 @default.
• W2016274034 hasOpenAccess W2016274034 @default.
• W2016274034 hasPrimaryLocation W20162740341 @default.
• W2016274034 hasRelatedWork W1497376611 @default.
• W2016274034 hasRelatedWork W1988070079 @default.
• W2016274034 hasRelatedWork W2033279904 @default.
• W2016274034 hasRelatedWork W2276041758 @default.
• W2016274034 hasRelatedWork W2282739671 @default.
• W2016274034 hasRelatedWork W2353682894 @default.
• W2016274034 hasRelatedWork W2368994920 @default.
• W2016274034 hasRelatedWork W2481078160 @default.
• W2016274034 hasRelatedWork W2516647071 @default.

• next