FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016280780> ?p ?o ?g. }

• W2016280780 endingPage "2744" @default.
• W2016280780 startingPage "2735" @default.
• W2016280780 abstract "Kynureninase is a member of a large family of catalytically diverse but structurally homologous pyridoxal 5‘-phosphate (PLP) dependent enzymes known as the aspartate aminotransferase superfamily or α-family. The Homo sapiens and other eukaryotic constitutive kynureninases preferentially catalyze the hydrolytic cleavage of 3-hydroxy-l-kynurenine to produce 3-hydroxyanthranilate and l-alanine, while l-kynurenine is the substrate of many prokaryotic inducible kynureninases. The human enzyme was cloned with an N-terminal hexahistidine tag, expressed, and purified from a bacterial expression system using Ni metal ion affinity chromatography. Kinetic characterization of the recombinant enzyme reveals classic Michaelis−Menten behavior, with a Km of 28.3 ± 1.9 μM and a specific activity of 1.75 μmol min-1 mg-1 for 3-hydroxy-dl-kynurenine. Crystals of recombinant kynureninase that diffracted to 2.0 Å were obtained, and the atomic structure of the PLP-bound holoenzyme was determined by molecular replacement using the Pseudomonas fluorescens kynureninase structure (PDB entry 1qz9) as the phasing model. A structural superposition with the P. fluorescens kynureninase revealed that these two structures resemble the “open” and “closed” conformations of aspartate aminotransferase. The comparison illustrates the dynamic nature of these proteins' small domains and reveals a role for Arg-434 similar to its role in other AAT α-family members. Docking of 3-hydroxy-l-kynurenine into the human kynureninase active site suggests that Asn-333 and His-102 are involved in substrate binding and molecular discrimination between inducible and constitutive kynureninase substrates." @default.
• W2016280780 created "2016-06-24" @default.
• W2016280780 creator A5018034473 @default.
• W2016280780 creator A5027717438 @default.
• W2016280780 creator A5041242705 @default.
• W2016280780 creator A5043322135 @default.
• W2016280780 date "2007-02-15" @default.
• W2016280780 modified "2023-10-17" @default.
• W2016280780 title "Crystal Structure of <i>Homo sapiens</i> Kynureninase^," @default.
• W2016280780 cites W1516505350 @default.
• W2016280780 cites W1520730837 @default.
• W2016280780 cites W1539796472 @default.
• W2016280780 cites W1565057341 @default.
• W2016280780 cites W1849215389 @default.
• W2016280780 cites W1861199552 @default.
• W2016280780 cites W1935845881 @default.
• W2016280780 cites W1973183626 @default.
• W2016280780 cites W1974271031 @default.
• W2016280780 cites W1987547165 @default.
• W2016280780 cites W1987854300 @default.
• W2016280780 cites W1988585084 @default.
• W2016280780 cites W1988691690 @default.
• W2016280780 cites W1997927933 @default.
• W2016280780 cites W2001641653 @default.
• W2016280780 cites W2007257481 @default.
• W2016280780 cites W2008171507 @default.
• W2016280780 cites W2009107445 @default.
• W2016280780 cites W2013414388 @default.
• W2016280780 cites W2015642465 @default.
• W2016280780 cites W2019394625 @default.
• W2016280780 cites W2019540758 @default.
• W2016280780 cites W2028754843 @default.
• W2016280780 cites W2038840577 @default.
• W2016280780 cites W2038992595 @default.
• W2016280780 cites W2041656963 @default.
• W2016280780 cites W2043486236 @default.
• W2016280780 cites W2043736935 @default.
• W2016280780 cites W2043855224 @default.
• W2016280780 cites W2044507120 @default.
• W2016280780 cites W2049667196 @default.
• W2016280780 cites W2054572399 @default.
• W2016280780 cites W2055417862 @default.
• W2016280780 cites W2065173416 @default.
• W2016280780 cites W2066701330 @default.
• W2016280780 cites W2069294285 @default.
• W2016280780 cites W2074986801 @default.
• W2016280780 cites W2074988177 @default.
• W2016280780 cites W2075010970 @default.
• W2016280780 cites W2077494556 @default.
• W2016280780 cites W2085721157 @default.
• W2016280780 cites W2097493124 @default.
• W2016280780 cites W2103365659 @default.
• W2016280780 cites W2106246537 @default.
• W2016280780 cites W2120276500 @default.
• W2016280780 cites W2123110132 @default.
• W2016280780 cites W2128046071 @default.
• W2016280780 cites W2128635872 @default.
• W2016280780 cites W2129993012 @default.
• W2016280780 cites W2130479394 @default.
• W2016280780 cites W2135839939 @default.
• W2016280780 cites W2139632794 @default.
• W2016280780 cites W2140616356 @default.
• W2016280780 cites W2144390151 @default.
• W2016280780 cites W2146087465 @default.
• W2016280780 cites W2152302193 @default.
• W2016280780 cites W2153134580 @default.
• W2016280780 cites W2157165549 @default.
• W2016280780 cites W2407927950 @default.
• W2016280780 cites W2969273703 @default.
• W2016280780 cites W4235403728 @default.
• W2016280780 cites W4299550713 @default.
• W2016280780 doi "https://doi.org/10.1021/bi0616697" @default.
• W2016280780 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2531291" @default.
• W2016280780 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17300176" @default.
• W2016280780 hasPublicationYear "2007" @default.
• W2016280780 type Work @default.
• W2016280780 sameAs 2016280780 @default.
• W2016280780 citedByCount "42" @default.
• W2016280780 countsByYear W20162807802012 @default.
• W2016280780 countsByYear W20162807802013 @default.
• W2016280780 countsByYear W20162807802014 @default.
• W2016280780 countsByYear W20162807802015 @default.
• W2016280780 countsByYear W20162807802016 @default.
• W2016280780 countsByYear W20162807802017 @default.
• W2016280780 countsByYear W20162807802019 @default.
• W2016280780 countsByYear W20162807802020 @default.
• W2016280780 countsByYear W20162807802021 @default.
• W2016280780 countsByYear W20162807802022 @default.
• W2016280780 crossrefType "journal-article" @default.
• W2016280780 hasAuthorship W2016280780A5018034473 @default.
• W2016280780 hasAuthorship W2016280780A5027717438 @default.
• W2016280780 hasAuthorship W2016280780A5041242705 @default.
• W2016280780 hasAuthorship W2016280780A5043322135 @default.
• W2016280780 hasBestOaLocation W20162807802 @default.
• W2016280780 hasConcept C104317684 @default.
• W2016280780 hasConcept C181199279 @default.
• W2016280780 hasConcept C185592680 @default.
• W2016280780 hasConcept C2776706248 @default.

• next