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- W2016289403 abstract "An examination of fluorescein-labelled Erythrina cristigalli lectin (FITC-ECL) staining on feline mononuclear cells (MNC) using fluorescent microscopy and a novel sugar titration competition assay revealed that monocytes (MO) were stained brighter by FITC-ECL than were lymphocytes (LYM). When MNC were stained with FITC-ECL in the presence of 400 mM or greater D-galactose, analysis by flow cytometry revealed continued MO staining while LYM were negative. MO expressed a larger quantity of carbohydrate receptors (CHO-R) for ECL than did LYM. The CHO-R expressed on MO were mostly protease-insensitive and uncapped by sialic acid residues. All of the CHO-R on LYM were protease-sensitive and many were capped by sialic acid residues. A combined labelling of MNC for non-specific esterase staining, latex bead ingestion and FITC-ECL staining in the presence of 400 mM D-galactose confirmed that FITC-ECL specifically stains MO in the presence of high sugar competitor concentrations." @default.
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- W2016289403 date "1990-07-01" @default.
- W2016289403 modified "2023-09-25" @default.
- W2016289403 title "Sugar competition assays reveal high affinity receptors for Erythrina cristigalli lectin on feline monocytes" @default.
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- W2016289403 doi "https://doi.org/10.1016/0022-1759(90)90227-m" @default.
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